GST antibody

Catalog No. ABIN99862

Product Details anti-GST Antibody

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Goat

Clonality: Polyclonal

Conjugate: This GST antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.

Characteristics: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.

Sterility: Sterile filtered

Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
Immunogen Type: NativeProtein

Isotype: IgG

Application Details

Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This antibody has also been reported to be suitable for immobilization in Label-free Interaction Analysis (Biocore).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References for anti-GST antibody (ABIN99862)

Licht, Hartl, Amman, Anrather, Janisiw, Jantsch: "Inosine induces context-dependent recoding and translational stalling." in: Nucleic acids research, Vol. 47, Issue 1, pp. 3-14, (2019) (PubMed).

Machida, Kubota, Kobayashi, Iwadate, Saeki, Yamaura, Nomura, Takiguchi, Hiwasa: "Identification of stroke-associated-antigens via screening of recombinant proteins from the human expression cDNA library (SEREX)." in: Journal of translational medicine, Vol. 13, pp. 71, (2016) (PubMed).

Hang, Lin, Grant, Fleurkens, Villiger, Soos, Morbidelli, Woods, Gauss, Aebi: "Analysis of site-specific N-glycan remodeling in the endoplasmic reticulum and the Golgi." in: Glycobiology, Vol. 25, Issue 12, pp. 1335-49, (2016) (PubMed).

Pitoniak, Chavel, Chow, Smith, Camara, Karunanithi, Li, Wolfe, Cullen: "Cdc42p-interacting protein Bem4p regulates the filamentous-growth mitogen-activated protein kinase pathway." in: Molecular and cellular biology, Vol. 35, Issue 2, pp. 417-36, (2015) (PubMed).

Karunanithi, Cullen: "The filamentous growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 transcriptional repressors in Saccharomyces cerevisiae." in: Genetics, Vol. 192, Issue 3, pp. 869-87, (2013) (PubMed).

Rizzolio, Lucchetti, Caligiuri, Marchesi, Caputo, Klein-Szanto, Bagella, Castronovo, Giordano: "Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1." in: Cell death and differentiation, Vol. 19, Issue 7, pp. 1152-61, (2012) (PubMed).

Sagermann, Chapleau, DeLorimier, Lei: "Using affinity chromatography to engineer and characterize pH-dependent protein switches." in: Protein science : a publication of the Protein Society, Vol. 18, Issue 1, pp. 217-28, (2009) (PubMed).

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: GST (GST Products)

Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
Synonyms: GST, Glutathione-S-Transferase

UniProt: P08515