anti-Goat KRT18 antibody for Western Blotting

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • krtt1c6
  • zgc:66015
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18 pseudogene
  • keratin, type I cytoskeletal 18
  • keratin, type I cytoskeletal 18-like
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • zgc:77517
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC693413
  • LOC700110
  • LOC700569
  • LOC704517
  • LOC100136778
  • k1c18
  • LOC100008885
  • zgc:77517
Reactivity
Cat (Feline), Cow (Bovine), Dog (Canine), Ferret, Goat, Hamster, Horse (Equine), Human, Marmoset, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
944
200
170
68
63
41
34
33
26
25
22
20
17
15
11
6
6
5
4
4
3
2
2
2
1
1
1
Host
Mouse
695
275
12
11
4
3
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
52
48
22
18
17
17
16
14
14
14
14
14
14
14
14
11
11
8
4
3
3
3
3
3
3
3
3
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
654
465
385
345
330
300
222
177
126
40
26
18
18
14
11
9
3
2
2
1
1
1
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Catalog No. ABIN3026966
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3026968 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see C-04 or Ks18-04

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Cat (Feline), Cow (Bovine), Dog (Canine), Ferret, Goat, Hamster, Horse (Equine), Human, Marmoset, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(944), (200), (170), (68), (63), (41), (34), (33), (26), (25), (22), (20), (17), (15), (11), (6), (6), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(695), (275), (12), (11), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(52), (48), (22), (18), (17), (17), (16), (14), (14), (14), (14), (14), (14), (14), (14), (11), (11), (8), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(654), (465), (385), (345), (330), (300), (222), (177), (126), (40), (26), (18), (18), (14), (11), (9), (3), (2), (2), (1), (1), (1)
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling Images
No Cross-Reactivity Chicken, Xenopus laevis
Characteristics This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Purification Protein G affinity chromatography
Immunogen A cytoskeletal preparation from A431 cells was used as the immunogen for the Keratin 18 antibody.
Clone C-04 or Ks18-04
Isotype IgG1, kappa

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Keratin 18 (KRT18 Antibody Abstract)
Background This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling Images back to top
Application Notes Optimal dilution of the Keratin 18 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Keratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) antibody (ABIN3026966) Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Keratin 18 ant...