Protein L Resin

Details for Product No. ABIN1536561, Supplier: Log in to see
Peptostreptococcus magnus (P. magnus)
Affinity Chromatography (AC), Purification (Purif)
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Purpose Protein L Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
Specificity Ligand Highly purified protein L
Number of Ig binding sites per ligand 5
MW of ligand Approximately 42 kDa
PI of ligand 4.57
Degree of substitution Approximately 2 mg protein L/ml settled resin
Static binding capacity >15 mg rabbit IgG/ml settled resin
Matrix spherical Agarose, 4% cross-linked
Average particle size 90 μm (45 - 165 μm)
Storage solution 1×PBS containing 20% ethanol
Sanitization Washing of the packed column with 70% ethanol
Characteristics The highly purified protein L ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein L Resin is greater than 15 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Bead Ligand Protein L
Bead Matrix Agarose beads
Bead Size 90 µm
Background Protein L, first isolated from the surface of bacterial species Peptostreptoccus magnus, binds immunoglobulin through κ light chain interaction. Protein L binds a wider range of Ig classes and subclasses than other antibody-binding proteins such as protein A or protein G. It can bind to all classes of Ig (i.e., IgG, IgM, IgA, IgE, and IgD) and also the single chain variable fragments (Scfv) and Fab fragments. 

High temperature heating is not recommended. The agarose melts above 65°C.

Reagent Preparation

Water and chemicals used for buffer preparation should be of high purity. It is recommended filtering the buffers by passing them through a 0.45 μm filter before use.
Binding/Wash Buffer: 20 mM Na2HPO4, 0.15 M NaCl, pH 8.0
Elution Buffer: 0.1 M glycine, pH 2.5
Neutralization Buffer: 1 M Tris-HCl, pH 8.5

Sample Preparation

To insure that proper ionic strength and pH are maintained for optimal binding, it is necessary to dilute serum samples, ascite fluid or cell culture supernatant at least 1:1 with Binding/Wash Buffer. Alternatively, the sample may be dialyzed overnight against Binding/Wash Buffer.

Assay Procedure

Packing of Column
1) Resuspend completely the resin and transfer 1 ml slurry to a new column, in which 1 ml Binding/Wash Buffer was added in advance.
2) Allow the resin to settle down and the buffer to drain from the column.
3) Add 5 ml binding/Wash Buffer onto the column to equilibrate the resin and drain the buffer with a flow speed of about 1 ml/min.

Column Purification
1) Apply the sample onto the column and drain the flow-through with a flow speed of about 1 ml/min. Collect the flow-through for measuring the binding efficiency to the resin, i.e. by SDS-PAGE.
2) Wash the column with 30 ml Binding/Wash Buffer and drain the buffer with a flow speed of about 2 ml/min, or until the absorbance of the effluent at 280 nm is stable.
3) Elute the immunoglobulins with 10-15 ml Elution Buffer and drain the eluate with a flow speed of about 1 ml/min. Collect the eluate and immediately neutralize to pH 7.4 with Neutralization Buffer (1/10 volume of total eluate)

Regeneration of Column
Regenerate the column by washing the resin with 10 ml Elution Buffer followed by equilibration with 5 ml Binding/Wash Buffer. Columns can be regenerated up to 10 times without significant loss of binding capacity.

Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store regenerated Protein L Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.
Expiry Date 18 months
Product cited in: Safdari, Farajnia, Asgharzadeh, Omidfar, Khalili: "humMR1, a highly specific humanized single chain antibody for targeting EGFRvIII." in: International immunopharmacology, Vol. 18, Issue 2, pp. 304-10, 2014 (PubMed).

De Oliveira, Wang, Ryan, Morrison, Kohn, Hollis: "A CD19/Fc fusion protein for detection of anti-CD19 chimeric antigen receptors." in: Journal of translational medicine, Vol. 11, pp. 23, 2013 (PubMed).

Zhang, Mao, Cao, Xiong, Wen, Chen, Zhu: "Generation and characterization of a novel recombinant antibody against LMP1-TES1 of Epstein-Barr virus isolated by phage display." in: Viruses, Vol. 5, Issue 4, pp. 1131-42, 2013 (PubMed).

Lin, Zhang, Wang, Lu, Zheng, Wang, Tang, Xu, Chen, Zhang, Zhao, Zhu, Mao, Feng: "A novel human Fab antibody for Trop2 inhibits breast cancer growth in vitro and in vivo." in: International journal of cancer. Journal international du cancer, Vol. 134, Issue 5, pp. 1239-49, 2013 (PubMed).

Chen, Zhang, Mao, Zhu, Ming, Wen, Ma, Cao, Lin, Tang, Liang, Feng: "A human Fab-based immunoconjugate specific for the LMP1 extracellular domain inhibits nasopharyngeal carcinoma growth in vitro and in vivo." in: Molecular cancer therapeutics, Vol. 11, Issue 3, pp. 594-603, 2012 (PubMed).

Rouet, Lowe, Dudgeon, Roome, Schofield, Langley, Andrews, Whitfeld, Jermutus, Christ: "Expression of high-affinity human antibody fragments in bacteria." in: Nature protocols, Vol. 7, Issue 2, pp. 364-73, 2012 (PubMed).

Wang, Zhang, Liu, Chen, Feng, Shang, Maziarz, Radtke, Hampe: "Anti-idiotypic antibody specific to GAD65 autoantibody prevents type 1 diabetes in the NOD mouse." in: PLoS ONE, Vol. 7, Issue 2, pp. e32515, 2012 (PubMed).

Dudgeon, Rouet, Kokmeijer, Schofield, Stolp, Langley, Stock, Christ: "General strategy for the generation of human antibody variable domains with increased aggregation resistance." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 27, pp. 10879-84, 2012 (PubMed).