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Protein A Resin

Purif, AC Protein A Agarose beads 90 µm
Catalog No. ABIN1536564
  • Target See all Protein A products
    Protein A
    Staphylococcus aureus
    Purification (Purif), Affinity Chromatography (AC)
    Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
    Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

    Ligand: Recombinant Streptococcal protein A expressed in E. coli
    Number of IgG binding sites per ligand: 5
    MW of ligand: Approximately 34 kDa
    PI of ligand: 5.17
    Degree of substitution: Approximately 2 mg protein A/ml settled resin
    Static binding capacity: >20 mg rabbit IgG/ml settled resin
    Matrix: spherical agarose, 4%
    Average particle size: 90 μm (45-165 μm)
    Bead Ligand
    Protein A
    Bead Matrix
    Agarose beads
    Bead Size
    90 µm
  • Comment

    High temperature heating is not recommended. The agarose melts above 65°C.

    For Research Use only
  • Format
    1X PBS containing 20% ethanol
    4 °C
    Storage Comment
    Store regenerated Protein A Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.
    Expiry Date
    18 months
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    Mui, Zhou, Blanchette, Chughtai, Knight, Gruosso, Papadakis, Huang, Park, Gingras, Branton: "The Human Adenovirus Type 5 E4orf4 Protein Targets Two Phosphatase Regulators of the Hippo Signaling Pathway." in: Journal of virology, Vol. 89, Issue 17, pp. 8855-70, (2015) (PubMed).

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    Asavapanumas, Ratelade, Verkman: "Unique neuromyelitis optica pathology produced in naïve rats by intracerebral administration of NMO-IgG." in: Acta neuropathologica, Vol. 127, Issue 4, pp. 539-51, (2014) (PubMed).

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    Wang, Zhao, Wang: "C-type lectin binds to ?-integrin to promote hemocytic phagocytosis in an invertebrate." in: The Journal of biological chemistry, Vol. 289, Issue 4, pp. 2405-14, (2014) (PubMed).

    Mabashi-Asazuma, Kuo, Khoo, Jarvis: "A novel baculovirus vector for the production of nonfucosylated recombinant glycoproteins in insect cells." in: Glycobiology, Vol. 24, Issue 3, pp. 325-40, (2014) (PubMed).

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    Asavapanumas, Verkman: "Neuromyelitis optica pathology in rats following intraperitoneal injection of NMO-IgG and intracerebral needle injury." in: Acta neuropathologica communications, Vol. 2, pp. 48, (2014) (PubMed).

    Payapilly, High: "BAG6 regulates the quality control of a polytopic ERAD substrate." in: Journal of cell science, Vol. 127, Issue Pt 13, pp. 2898-909, (2014) (PubMed).

    Gow, Sauter, Pridans, Moffat, Sehgal, Stutchfield, Raza, Beard, Tsai, Bainbridge, Boner, Fici, Garcia-Tapia, Martin, Oliphant, Shelly, Tiwari, Wilson, Smith, Mabbott, Hume: "Characterisation of a novel Fc conjugate of macrophage colony-stimulating factor." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 22, Issue 9, pp. 1580-92, (2014) (PubMed).

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    Song, Kim, Kim, Lee, Kim, Park: "IGFBP5 mediates high glucose-induced cardiac fibroblast activation." in: Journal of molecular endocrinology, Vol. 50, Issue 3, pp. 291-303, (2013) (PubMed).

  • Target
    Protein A
    Protein A Products
    alpha protein Bead, repB Bead
    Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens. 
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