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Co-IP, IP, Purif, ChIP, RIP Antibody Agarose beads 90 μm
Catalog No. ABIN5311510
  • Key Features
    • High-affinity single-domain antibody
    • Recognizes mRFP, mCherry, mScarlet-i, tdTomato, dsRed etc.
    • Bead size 50-150 μm
    Target See all RFP products
    RFP (Red Fluorescent Protein (RFP))
    Camelid (Camelidae)
    Protein Complex Immunoprecipitation (Co-IP), Immunoprecipitation (IP), Purification (Purif), Chromatin Immunoprecipitation (ChIP), RNA-Binding Protein Immunoprecipitation (RIP)
    RFP-Catcher is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.
    Sample Type
    Cell Extracts
    Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
    Cross-Reactivity (Details)
    Does not cross-react with GFP or mTagBFP derivatives.
    RFP-Catcher is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4 % cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer.
    RFP-Catcher thus features high affinity and superior capacity for RFP fusion proteins while showing negligible non-specific background.
    RFP-Catcher immobilizes a wide range of RFP derivatives including mCherry and mScarlet.
    RFP Selector is compatible not only with physiological buffers but also with high stringency buffers.
    RFP-Catcher thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
    4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody
    Material not included
    wash buffers, columns, tubes
    Bead Ligand
    Bead Matrix
    Agarose beads
    Bead Size
    90 μm
  • Application Notes
    Coating: sdAb anti-RFP clone 2B12
    Matrix: 4 % cross-linked agarose, bead size 50-150 μm
    Capacity: > 4 μg RFP per μl of packed beads (= 2 μL of slurry)
    Buffer Compatibility:
    • Common buffer substances at pH 5 to 9
    • 2 % Triton X-100, 1 % Tween-20, 1 % NP-40, 1 % CHAPS, 1 % Deoxycholate, 0.1 % SDS
    • 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA
    • 4 M urea
    • 10 mM DTT, 10 mM 2-Mercaptoethanol
    • RNAse A, DNAse I, Benzonase, protease inhibitors
    For Research Use only
  • Buffer
    50 % slurry in PBS containing 20 % Ethanol
    4 °C
    Storage Comment
    Store at 4 °C, do not freeze
    Expiry Date
    12 months
  • Target
    RFP (Red Fluorescent Protein (RFP))
    Alternative Name
    RFP (RFP Products)
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