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- Key Features
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- High-affinity single-domain antibody
- Recognizes mRFP, mCherry, mScarlet-i, tdTomato, dsRed etc.
- Bead size 50-150 μm
- Target See all RFP products
- RFP (Red Fluorescent Protein (RFP))
- Reactivity
- Discosoma
- Host
- Camelid (Camelidae)
- Application
- Protein Complex Immunoprecipitation (Co-IP), Immunoprecipitation (IP), Purification (Purif), Chromatin Immunoprecipitation (ChIP), RNA-Binding Protein Immunoprecipitation (RIP)
- Purpose
- RFP-Catcher is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.
- Sample Type
- Cell Extracts
- Specificity
- Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
- Cross-Reactivity (Details)
- Does not cross-react with GFP or mTagBFP derivatives.
- Characteristics
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RFP-Catcher is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4 % cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer.
RFP-Catcher thus features high affinity and superior capacity for RFP fusion proteins while showing negligible non-specific background.
RFP-Catcher immobilizes a wide range of RFP derivatives including mCherry and mScarlet.
RFP Selector is compatible not only with physiological buffers but also with high stringency buffers.
RFP-Catcher thus provides great freedom to adjust the binding and washing conditions to the experimental needs. - Components
- 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody
- Material not included
- wash buffers, columns, tubes
- Bead Ligand
- Antibody
- Bead Matrix
- Agarose beads
- Bead Size
- 90 μm
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- Application Notes
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Coating: sdAb anti-RFP clone 2B12
Matrix: 4 % cross-linked agarose, bead size 50-150 μm
Capacity: > 4 μg RFP per μl of packed beads (= 2 μL of slurry)
Buffer Compatibility:- Common buffer substances at pH 5 to 9
- 2 % Triton X-100, 1 % Tween-20, 1 % NP-40, 1 % CHAPS, 1 % Deoxycholate, 0.1 % SDS
- 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA
- 4 M urea
- 10 mM DTT, 10 mM 2-Mercaptoethanol
- RNAse A, DNAse I, Benzonase, protease inhibitors
- Restrictions
- For Research Use only
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- Buffer
- 50 % slurry in PBS containing 20 % Ethanol
- Storage
- 4 °C
- Storage Comment
- Store at 4 °C, do not freeze
- Expiry Date
- 12 months
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- Target
- RFP (Red Fluorescent Protein (RFP))
- Alternative Name
- RFP (RFP Products)
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