Cell Staining Buffer

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Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Characteristics Cell Staining Buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell suspensions. Cell Staining Buffer contains bovine calf serum as a protein carrier to reduce non-specific binding of antibodies and fluorochrome reagents to target cells. It also contains a metabolic inhibitor, sodium azide (NaN3), to inhibit patching and capping of cell surface antigens. To prevent interference in biotin/avidin indirect staining protocols, Cell Staining Buffer is formulated without biotin.
Components Cell Staining Buffer
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store between 2°C and 8°C.
Background publications Dong, Wodziak, Lowe: "Epidermal growth factor receptor (EGFR) signaling requires a specific endoplasmic reticulum thioredoxin for the post-translational control of receptor presentation to the cell surface." in: The Journal of biological chemistry, Vol. 290, Issue 13, pp. 8016-27, 2015 (PubMed).

de Vries, Heijink, Gras, den Boef, Reinders-Luinge, Pouwels, Hylkema, van der Toorn, Brouwer, van Oosterhout, Nawijn: "Pim1 kinase protects airway epithelial cells from cigarette smoke-induced damage and airway inflammation." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 307, Issue 3, pp. L240-51, 2014 (PubMed).

Kusner, Ciesielski, Marx, Kaminski, Fenstermaker: "Survivin as a potential mediator to support autoreactive cell survival in myasthenia gravis: a human and animal model study." in: PLoS ONE, Vol. 9, Issue 7, pp. e102231, 2014 (PubMed).

Garcia, Pino, Mizutani, Cardona, Charo, Ransohoff, Forsthuber, Cardona: "Regulation of adaptive immunity by the fractalkine receptor during autoimmune inflammation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 3, pp. 1063-72, 2013 (PubMed).

Murali, Ornelles, Gooding, Wilms, Huang, Tollefson, Wold, Garnett-Benson: "Adenovirus death protein (ADP) is required for lytic infection of human lymphocytes." in: Journal of virology, Vol. 88, Issue 2, pp. 903-12, 2013 (PubMed).

Yu, Ware, Waterland, Zhang, Chen, Gadkari, Kunde-Ramamoorthy, Nosavanh, Shen: "Developmentally programmed 3' CpG island methylation confers tissue- and cell-type-specific transcriptional activation." in: Molecular and cellular biology, Vol. 33, Issue 9, pp. 1845-58, 2013 (PubMed).