Rabbit IgG Isotype Control

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ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Isotype IgG
Purification Purified by Protein A.

Isotype controls are negative controls that are designed to measure the amount of non-specific background signal caused by the primary antibody. Isotype Control have no specificity for the target protein, yet retain all of the non-specific characteristics of an antibody.

Molecular Weight 150 kDa
Application Notes WB(Assay-dependent)
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Product cited in: Xia, Jiao: "Histone variant H3.3 orchestrates neural stem cell differentiation in the developing brain." in: Cell death and differentiation, Vol. 24, Issue 9, pp. 1548-1563, 2017 (PubMed).

Chen, Qu, Li, Ye, Zhang, Qin, Fan: "Effects of 1,25-dihydroxyvitamin D3 in an ovalbumin-induced allergic rhinitis model." in: International immunopharmacology, Vol. 47, pp. 182-189, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)). Further details: Sample: Nasopharynx-associated lymphoid tissue (

Miyabe, Miyabe, Murooka, Kim, Newton, Kim, Haribabu, Luscinskas, Mempel, Luster: "Complement C5a Receptor is the Key Initiator of Neutrophil Adhesion Igniting Immune Complex-induced Arthritis." in: Science immunology, Vol. 2, Issue 7, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Wang, Wang, Zhu, Long, Yan: "Vitamin K2 can suppress the expression of Toll-like receptor 2 (TLR2) and TLR4, and inhibit calcification of aortic intima in ApoE-/-mice as well as smooth muscle cells." in: Vascular, Vol. 26, Issue 1, pp. 18-26, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: heart

Zhao, Yang, Zhou, Zhang, Cheng, Huang, Zhang, Wang, Wang, Chen, Zhu, Li: "Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 35, pp. E7245-E7254, 2017 (PubMed).

Braun, Khan, Khan, Kumar, Ward, Achyut, Arbab, Hess, Hoda, Baban, Dhandapani, Vaibhav: "Selective activation of cannabinoid receptor-2 reduces neuroinflammation after traumatic brain injury via alternative macrophage polarization." in: Brain, behavior, and immunity, Vol. 68, pp. 224-237, 2017 (PubMed).

Christensen, Juhl, Persson, Charabi, Mortensen, Kiss, Lelkaitis, Rubek, von Buchwald, Kjær: "uPAR-targeted optical near-infrared (NIR) fluorescence imaging and PET for image-guided surgery in head and neck cancer: proof-of-concept in orthotopic xenograft model." in: Oncotarget, Vol. 8, Issue 9, pp. 15407-15419, 2016 (PubMed). Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Human).

Zhang, Zhao, Lin, Che, Li, Jiang, Hu, Wang: "Role of PTX3 in corneal epithelial innate immunity against Aspergillus fumigatus infection." in: Experimental eye research, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Yang, Jiang: "Embedding Raman Tags between Au Nanostar@Nanoshell for Multiplex Immunosensing." in: Small (Weinheim an der Bergstrasse, Germany), Vol. 12, Issue 36, pp. 4980-4985, 2016 (PubMed).

Brestoff, Kim, Saenz, Stine, Monticelli, Sonnenberg, Thome, Farber, Lutfy, Seale, Artis: "Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity." in: Nature, 2015 (PubMed). (Sample species: Mouse (Murine)).

Benesch, Ko, Tang, Dewald, Lopez-Campistrous, Zhao, Lai, Curtis, Brindley, McMullen: "Autotaxin is an inflammatory mediator and therapeutic target in thyroid cancer." in: Endocrine-related cancer, Vol. 22, Issue 4, pp. 593-607, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: thyroid cancer

Benesch, Tang, Dewald, Dong, Mackey, Hemmings, McMullen, Brindley: "Tumor-induced inflammation in mammary adipose tissue stimulates a vicious cycle of autotaxin expression and breast cancer progression." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 9, pp. 3990-4000, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: breast cancer tissue

Chenna Narendra, Chalise, Magnusson, Uppugunduri: "Local but Not Systemic Administration of Uridine Prevents Development of Antigen-Induced Arthritis." in: PLoS ONE, Vol. 10, Issue 10, pp. e0141863, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Shang, Fu, Cheng, Wang, Liu, Zhao, Gu: "Photonic Crystal Microbubbles as Suspension Barcodes." in: Journal of the American Chemical Society, Vol. 137, Issue 49, pp. 15533-9, 2015 (PubMed).

Lee, Bae, Choi, Park, Chun: "Molecular cloning and expression analysis of pig CD7." in: Veterinary research communications, Vol. 38, Issue 3, pp. 257-63, 2014 (PubMed). (Sample species: Pig (Porcine)).

Zhuang, Hu, Tan, Zhu, Liu, Liu, Yan, Lin: "Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells." in: Biochimie, Vol. 105, pp. 91-8, 2014 (PubMed).

Zhou, Xu, Zhang, Feng, Zhang, Zou, Liao: "Natural IgG antibodies in normal rabbit serum are involved in killing of the ompP2 mutant of Haemophilus parasuis SC096 strain via the classical complement pathway." in: Veterinary journal (London, England : 1997), Vol. 196, Issue 1, pp. 111-3, 2013 (PubMed). (Sample species: Others).

Huang, He, Gan, Ma: "Solidified liquid layer model makes quartz crystal microbalance a convenient molecular ruler." in: Colloids and surfaces. B, Biointerfaces, Vol. 85, Issue 1, pp. 92-6, 2011 (PubMed).

Ma, He, Liu, Gan, Jin, Zhou: "Surface initiated polymerization from substrates of low initiator density and its applications in biosensors." in: ACS applied materials & interfaces, Vol. 2, Issue 11, pp. 3223-30, 2010 (PubMed).