BCP Albumin Assay Kit

Catalog No. ABIN1000255

Product Details

Target: BCP Albumin

Application: Biochemical Assay (BCA)

Sample Type: Serum, Urine

Specificity: 0.3 g/dL

Characteristics: Sensitive and accurate. Use as little as 20 µL samples. Detection range 0.3 g/dL (45µM) to 5 g/dL (750µM) albumin in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
Low interference in biological samples.
No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein.

Components: Reagent: 50 mL. Albumin standard: 2 mL 5 g/dL BSA.

Material not included: Pipeting devices and accessories (e.g. 5 µL). Clear bottom 96-well plates (e.g. Corning Costar) and plate reader. Spectrophotometer and cuvets for measuring OD at 610nm.

Application Details

Application Notes: Direct Assays: albumin in serum, urine, biological preparations.
Drug Discovery/Pharmacology: effects of drugs on albumin metabolism.

Protocol: Procedure using 96-well plate:
1. Dilute standards in distilled water. Dilute serum samples 2 fold. Transfer 20 µL diluted standards and diluted samples to wells of a clear bottom plate. Store diluted standards at -20°C for future use.
2. Add 200 µL working reagent and tap lightly to mix. Avoid bubble formation!
3. Incubate 5 min at room temperature and read optical density at 590- 630nm (peak absorbance at 610nm).

Procedure using cuvette:
1. Transfer 60 µL Blank, Standards and samples to appropriately labeled tubes. Add 1000 µL working reagent and tap lightly to mix. Incubate 5 min at room temperature.
2. Transfer to cuvet and read optical density at 610nm. Important: if sample OD is higher than the OD for standard, dilute samples in distilled water and repeat the assay.

Reagent Preparation:

Important: bring reagent to room temperature before use.

Calculation of Results:

Subtract blank OD (water, #8) from the standard OD values and plot the OD against standard concentrations. Use the standard curve to determine the sample albumin concentration.
Conversions: 0.1 g/dL albumin equals 15 µM, 0.1% or 1000 ppm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References

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Shin, Choi, Hur, Lee, Kim, Cho, Park: "Local production of total IgE and specific antibodies to the house dust mite in adenoid tissue." in: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology, Vol. 20, Issue 2, pp. 134-41, (2009) (PubMed).

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Shin, Choi, Park, Lee, Kim, Cho: "Immunological investigation in the adenoid tissues from children with chronic rhinosinusitis." in: Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, Vol. 141, Issue 1, pp. 91-6, (2009) (PubMed).

Yung, Zhang, Zhang, Chan, Lui, Chan: "Anti-DNA antibody induction of protein kinase C phosphorylation and fibronectin synthesis in human and murine lupus and the effect of mycophenolic acid." in: Arthritis and rheumatism, Vol. 60, Issue 7, pp. 2071-82, (2009) (PubMed).

Maier, Gross, Hamlin, Maier, Workman, Kim-Howard, Schoeb, Farris: "Proteinuria of nonautoimmune origin in wild-type FVB/NJ mice." in: Comparative medicine, Vol. 57, Issue 3, pp. 255-66, (2007) (PubMed).

Target Details

Target: BCP Albumin

Background: Quantitative determination of albumin by bromcresol purple BCP method at 610nm.
Procedure: 5 min.

Albumin is the most abundant plasma protein in human. It accounts for about 60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure, binding of key substances such as long-chain fatty acids, bile acids, bilirubin, haematin, calcium, magnesium. It has anti-oxidant and anticoagulant effects, and also acts as a carrier for nutritional factors and drugs, as an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition and protein-losing enteropathies. High levels are associated with dehydration. Simple, direct and automation-ready procedures for measuring albumin concentration in biological samples are becoming popular in Research and Drug Discovery. This BCP albumin assay kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol purple that forms a colored complex specifically with albumin. The intensity of the color, measured at 610nm, is directly proportional to the albumin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.