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Phospholipase D Assay Kit

Catalog No. ABIN1000328
Plus shipping costs $45.00
100 tests
Shipping to: United States
Delivery in 4 to 6 Business Days
  • Target See all Phospholipase D (PLD) Kits
    Phospholipase D (PLD)
    Activity Assay (AcA)
    0.04 U/L
    Sensitive. Use 10 µL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.
    Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.
    Assay Buffer: 10 mL. Dye Reagent: 120 µL. Enzyme Mix: 120 µL. Substrate: 1.5 mL. Calibrator: 400 µL.
    Material not included
    Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.
  • Application Notes
    Direct Assays: phospholipase D in biological samples.
    Drug Discovery/Pharmacology: effects of drugs on phospholipase D metabolism.
    Calculation of Results

    Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.

    For Research Use only
  • Storage
    -20 °C
  • Target
    Phospholipase D (PLD)
    Alternative Name
    Phospholipase D (PLD Products)
    CG12110 Kit, Dmel\\CG12110 Kit, PLD Kit, dPLD Kit, dPld Kit, pld Kit, pld1 Kit, PHOSPHOLIPASE D Kit, PHOSPHOLIPASE D P1 Kit, PHOSPHOLIPASE D ZETA 1 Kit, PHOSPHOLIPASE D ZETA1 Kit, PLD ZETA 1 Kit, PLDZ1 Kit, PLDZETA1 Kit, phospholipase D P1 Kit, Phospholipase D Kit, phospholipase D P1 Kit, phospholipase Kit, phospholipase D Kit, phospholipase d Kit, glycosylphosphatidylinositol specific phospholipase D1 Kit, Pld Kit, PLDP1 Kit, RR_RS10410 Kit, CNE01480 Kit, pld Kit, plD Kit, GPLD1 Kit
    Quantitative determination of phospholipase D activity by colorimetric (570nm) or fluorimetric (530/590nm) method.
    Procedure: 30 min.

    Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. This method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.
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