MMP13 ELISA Kit

Catalog No. ABIN1112762

Product Details MMP13 ELISA Kit

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78-5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Analytical Method: Quantitative

Sensitivity: < 5 pg/mL

Components: 1. One 96-well plate pre-coated with anti-human MMP13 antibody 2. Lyophilized human MMP13 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-human MMP13 antibody (Concentrated): 130 µl.

Material not included: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Application Details

Comment:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP13 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP13 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the MMP13 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of MMP13 can be calculated.

Plate: Pre-coated

Reagent Preparation:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Sample Preparation:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysates and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin or EDTA as the anticoagulant. Centrifuge for 30 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide, Thimerosal (Merthiolate)

Target Details for MMP13

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: MMP-13 (MMP13 Products)

Synonyms: CLG3 ELISA Kit, MANDP1 ELISA Kit, Clg ELISA Kit, MMP-13 ELISA Kit, Mmp1 ELISA Kit, cb1034 ELISA Kit, mmp13 ELISA Kit, gene A ELISA Kit, xCol ELISA Kit, xcl3 ELISA Kit, MMP13 ELISA Kit, MGC108008 ELISA Kit, matrix metallopeptidase 13 ELISA Kit, matrix metallopeptidase 13a ELISA Kit, matrix metallopeptidase 13 (collagenase 3) like S homeolog ELISA Kit, matrix metallopeptidase 13 (collagenase 3) ELISA Kit, matrix metalloproteinase 13 ELISA Kit, matrix metallopeptidase 13 (collagenase 3) like L homeolog ELISA Kit, MMP13 ELISA Kit, Mmp13 ELISA Kit, mmp13a ELISA Kit, mmp13l.S ELISA Kit, mmp13 ELISA Kit, LOC100136348 ELISA Kit, mmp13l.L ELISA Kit

Background: Matrix metallopeptidase 13 (MMP13) is a member of the matrix metalloproteinase (MMP) family. Like most MMPs it secreated as an inactive pro-form. During embryonic development, MMP13 is expressed in the skeleton as required for restructuring the collagen matrix for bone mineralization. In pathological situations it is highly overexpressed, this occurs in human carcinomas and in rheumatoid arthritis and osteoarthritis. the expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition.