MMP13 ELISA Kit

Catalog No. ABIN921071

This Colorimetric ELISA kit is designed for the quantitative measurement of Human MMP13. There are 10 publications available.

Quick Overview for MMP13 ELISA Kit (ABIN921071)

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Binding Specificity: AA 20-471

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin)

Product Details MMP13 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich Human MMP13 ELISA Kit to quantitate Human MMP13 in cell culture supernatants, serum and plasma ( heparin).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: L20-C471

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 5 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.4%, 6.7%, 7%
Inter-Assay Precision (CV%): 7.5%, 8%, 8.1%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for MMP13 ELISA Kit (ABIN921071)

Khan, Haseeb, Ansari, Haqqi: "A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1β-induced activation of AP-1 in human OA chondrocytes." in: Scientific reports, Vol. 7, pp. 43789, (2018) (PubMed).

Nosratzehi, Alijani, Moodi: "Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma" in: Asian Pacific journal of cancer prevention : APJCP, Vol. 18, Issue 7, pp. 1947-1951, (2017) (PubMed).

Capsoni, Ongari, Lonati, Accetta, Gatti, Catania: "?-Melanocyte-stimulating-hormone (?-MSH) modulates human chondrocyte activation induced by proinflammatory cytokines." in: BMC musculoskeletal disorders, Vol. 16, pp. 154, (2015) (PubMed).

Agha-Hosseini, Mirzaii-Dizgah: "Serum and saliva collagenase-3 (MMP-13) in patients with oral lichen planus and oral squamous cell carcinoma." in: Medical journal of the Islamic Republic of Iran, Vol. 29, pp. 218, (2015) (PubMed).

Nikniaz, Ostadrahimi, Mahdavi, Ebrahimi, Nikniaz: "Effects of Elaeagnus angustifolia L. supplementation on serum levels of inflammatory cytokines and matrix metalloproteinases in females with knee osteoarthritis." in: Complementary therapies in medicine, Vol. 22, Issue 5, pp. 864-9, (2014) (PubMed).

Huang, Zou, Shi, Zhang, Pen, Zhang, Gao, Wang: "The effect of electroacupuncture on the extracellular matrix synthesis and degradation in a rabbit model of disc degeneration." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 731395, (2014) (PubMed).

Xu, Zhang, Zhao, Gao, Han, Su, Lei: "Phosphorylation of osteopontin in osteoarthritis degenerative cartilage and its effect on matrix metalloprotease 13." in: Rheumatology international, Vol. 33, Issue 5, pp. 1313-9, (2013) (PubMed).

Qin, He, Xia, Guo, He: "Effects of electro-acupuncture on oestrogen levels, body weight, articular cartilage histology and MMP-13 expression in ovariectomised rabbits." in: Acupuncture in medicine : journal of the British Medical Acupuncture Society, Vol. 31, Issue 2, pp. 214-21, (2013) (PubMed).

Yang, Gao, Rao, Shen: "Clinicopathological significance and prognostic value of MMP-13 expression in colorectal cancer." in: Scandinavian journal of clinical and laboratory investigation, Vol. 72, Issue 6, pp. 501-5, (2012) (PubMed).

Xia, Luo, Lin, Zhang, Guo, He: "The effect of different treatment time of millimeter wave on chondrocyte apoptosis, caspase-3, caspase-8, and MMP-13 expression in rabbit surgically induced model of knee osteoarthritis." in: Rheumatology international, Vol. 32, Issue 9, pp. 2847-56, (2012) (PubMed).

Target Details for MMP13

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: MMP13

Background:

Background: Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Gene Full Name: matrix metallopeptidase 13

Gene ID: 4322

UniProt: P45452