NGFB ELISA Kit (Nerve Growth Factor beta)

Details for Product NGFB ELISA Kit No. ABIN1112765, Supplier: Log in to see
  • NGFB
  • ngfb
  • ns:zft386
  • xx:zft386
  • zgc:109730
  • hsan5
  • beta-ngf
  • Ngfb
  • Beta-NGF
  • HSAN5
  • beta-NGF
  • nerve growth factor
  • nerve growth factor b (beta polypeptide)
  • beta-Nerve growth factor
  • nerve growth factor (beta polypeptide)
  • NGF
  • ngfb
  • FPV072
  • FPV076
  • ngf
  • Ngf
Mouse (Murine)
Kits with alternative reactivity to:
Method Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
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Supplier Product No.
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Analytical Method Quantitative
Detection Method Colorimetric
Sensitivity < 1 pg/mL
Components 1. One 96-well plate pre-coated with anti-mouse NGF antibody 2. Lyophilized Mouse NGF standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse NGF antibody (Concentrated): 130 µl.
Material not included 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
Alternative Name NGF (NGFB ELISA Kit Abstract)
Background Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF consists of 3 types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight complex. It is mainly expressed in Sertoli and interstitial cells. The proforms of NGF are secreted and cleaved extracellularly by the serine protease plasmin and by selective matrix metalloproteinases. NGF is important for the growth, maintenance, and survival of certain target neurons (nerve cells). It also functions as a signaling molecule.
Pathways NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-NGF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NGF polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the NGF amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of NGF can be calculated.

Plate Pre-coated
Reagent Preparation
  1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
Sample Preparation

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysates and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 2000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C . Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions For Research Use only
Preservative Sodium azide, Thimerosal (Merthiolate)
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