SAA1 ELISA Kit (Serum Amyloid A1)

Details for Product SAA1 ELISA Kit No. ABIN1889362
Target
Binding Specificity
AA 20-122
Reactivity
Mouse
Alternatives
Kits with alternative reactivity to:
4
4
3
2
2
2
1
1
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
1.56-100 ng/mL
Minimum Detection Limit
1.56 ng/mL
Application
ELISA
Options
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse SAA
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
Analytical Method Quantitative
Detection Method Colorimetric
Immunogen Expression system for standard: E.coli
Immunogen sequence: G20-Y122
Specificity Expression system for standard: E.coli
Immunogen sequence: G20-Y122
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <150pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Target
Alternative Name SAA1 (SAA1 ELISA Kit Abstract)
Background

Protein Function: Major acute phase protein. .

Background: Serum amyloid A protein(SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.

Synonyms: Serum amyloid A-1 protein,Saa1,

Full Gene Name: Serum amyloid A-1 protein

Cellular Localisation: Secreted.
Gene ID 111345
UniProt P05366
Pathways Toll-Like Receptors Cascades
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Tissue Specificity: Detected in blood plasma (at protein level). Detected in liver. .

Plate Pre-coated
Protocol mouse SAA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAA has been precoated onto 96-well plates. Standards(E.coli, G20-Y122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SAA amount of sample captured in plate.
Assay Procedure

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse SAA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse SAA standard solution and each sample be measured in duplicate.

Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 1.4, Standard deviation: 0.06, CV(%): 4.3
  • Sample 2: n=16, Mean(ng/ml): 3.4, Standard deviation: 0.177, CV(%): 5.2
  • Sample 3: n=16, Mean(ng/ml): 5.7, Standard deviation: 0.291, CV(%): 5.1,
  • Sample 1: n=24, Mean(ng/ml): 1.5, Standard deviation: 0.081, CV(%): 5.4
  • Sample 2: n=24, Mean(ng/ml): 3.6, Standard deviation: 0.223, CV(%): 6.2
  • Sample 3: n=24, Mean(ng/ml): 6.8, Standard deviation: 0.388, CV(%): 5.7
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months
Images
Image no. 1 for Serum Amyloid A1 (SAA1) ELISA Kit (ABIN1889362) Mouse SAA PicoKine ELISA Kit standard curve