ADAM12 ELISA Kit (ADAM Metallopeptidase Domain 12)
Details for Product ADAM12 ELISA Kit No. ABIN1889434- Antigen
-
show synonyms for this antigen
- ADAM12
- adam12
- Mltna
- mKIAA4001
- MCMP
- MCMPMltna
- MLTN
- MLTNA
- ADAM metallopeptidase domain 12
- a disintegrin and metallopeptidase domain 12 (meltrin alpha)
- ADAM12
- adam12
- Adam12
- Epitope
-
AA 29-513
- Reactivity
-
Mouse (Murine)
Alternatives - Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 156-10.000 pg/mL
- Minimum Detection Limit
- 156 pg/mL
- Application
-
ELISA
- Options
Application Notes | Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. |
Comment |
Sequence similarities: Contains 1 disintegrin domain. Tissue Specificity: Expressed during early developing mesenchymal cells that give rise to skeletal muscle, bones and visceral organs. Not expressed in adult normal muscle but expressed in regenerating muscle. . |
Plate | Pre-coated |
Protocol | mouse ADAM12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ADAM12 has been precoated onto 96-well plates. Standards(CHO, R29-S513) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ADAM12 amount of sample captured in plate. |
Assay Procedure |
Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse ADAM12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse ADAM12 standard solution and each sample be measured in duplicate. |
Assay Precision |
|
Restrictions | For Research Use only |
Handling Advice | Avoid multiple freeze-thaw cycles. |
Storage | -20 °C,4 °C |
Storage Comment | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles |
Expiry Date | 12 months |
Supplier Images |
Background publications |
Gilpin, Loechel, Mattei, Engvall, Albrechtsen, Wewer: "A novel, secreted form of human ADAM 12 (meltrin alpha) provokes myogenesis in vivo." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 157-66, 1998 (PubMed).
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