ADAM12 ELISA Kit (ADAM Metallopeptidase Domain 12)

Details for Product ADAM12 ELISA Kit No. ABIN1889434
Target
Binding Specificity
AA 29-513
Reactivity
Mouse
Alternatives
Kits with alternative reactivity to:
6
5
4
1
1
1
1
1
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
156-10.000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Options
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse ADAM12
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin)
Analytical Method Quantitative
Detection Method Colorimetric
Immunogen Expression system for standard: CHO
Immunogen sequence: R29-S513
Specificity Expression system for standard: CHO
Immunogen sequence: R29-S513
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Target
Alternative Name ADAM12 (ADAM12 ELISA Kit Abstract)
Background

Protein Function: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.

Background: ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

Synonyms: Disintegrin and metalloproteinase domain-containing protein 12,ADAM 12,3.4.24.-,Meltrin-alpha,Adam12,Mltna,

Full Gene Name: Disintegrin and metalloproteinase domain-containing protein 12

Cellular Localisation: Membrane, Single-pass type I membrane protein.
Gene ID 11489
UniProt Q61824
Pathways EGFR Signaling Pathway
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Contains 1 disintegrin domain.

Tissue Specificity: Expressed during early developing mesenchymal cells that give rise to skeletal muscle, bones and visceral organs. Not expressed in adult normal muscle but expressed in regenerating muscle. .
Plate Pre-coated
Protocol mouse ADAM12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ADAM12 has been precoated onto 96-well plates. Standards(CHO, R29-S513) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ADAM12 amount of sample captured in plate.
Assay Procedure

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse ADAM12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse ADAM12 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 1.45, Standard deviation: 0.062, CV(%): 4.3
  • Sample 2: n=16, Mean(ng/ml): 3.76, Standard deviation: 0.139, CV(%): 3.7
  • Sample 3: n=16, Mean(ng/ml): 6.87, Standard deviation: 0.24, CV(%): 3.5,
  • Sample 1: n=24, Mean(ng/ml): 1.54, Standard deviation: 0.089, CV(%): 5.8
  • Sample 2: n=24, Mean(ng/ml): 3.81, Standard deviation: 0.171, CV(%): 4.5
  • Sample 3: n=24, Mean(ng/ml): 7.02, Standard deviation: 0.365, CV(%): 5.2
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months
Images
Image no. 1 for ADAM Metallopeptidase Domain 12 (ADAM12) ELISA Kit (ABIN1889434) Mouse ADAM12 PicoKine ELISA Kit standard curve