RCBTB2 ELISA Kit (Regulator of Chromosome Condensation (RCC1) and BTB (POZ) Domain Containing Protein 2)

Details for Product RCBTB2 ELISA Kit No. ABIN2044389
Target
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
3
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.31-20 ng/mL
Minimum Detection Limit
0.31 ng/mL
Application
ELISA
Options
Brand BioAssay™
Detection Method Colorimetric
Sensitivity < 0.124 ng/mL
Target
Alternative Name RCC1 And BTB Domain Containing Protein 2 (RCBTB2) (RCBTB2 ELISA Kit Abstract)
Plate Pre-coated
Protocol RCC1 And BTB Domain Containing Protein 2 (RCBTB2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RCC1 And BTB Domain Containing Protein 2 (RCBTB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RCC1 And BTB Domain Containing Protein 2 (RCBTB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RCC1 And BTB Domain Containing Protein 2 (RCBTB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of RCC1 And BTB Domain Containing Protein 2 (RCBTB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C