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11,12-EET/DHET ELISA Kit

Reactivity: Chemical Colorimetric Cell Culture Cells, Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2648461
  • Target
    11,12-EET/DHET
    Reactivity
    Chemical
    Detection Method
    Colorimetric
    Application
    ELISA
    Sample Type
    Cell Culture Cells, Cell Culture Supernatant, Plasma, Serum
    Sensitivity
    10 pg/mL
    Characteristics
    It is well known that arachidonic acid (AA) will be converted to EET by P450 arachidonic acid epoxygenase (AA epoxygenase) and EET will be converted to DHET by soluble epoxide hydrolase (sEH) in vivo. Cytochrome P450 2J2 (CYP2J2) is a predominant human AA epoxygenase that produces all four EETs. The EET/DHET ELISA Assay kit can be used to measure EET levels in cultured cells which express sEH (1).
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Sample Volume
    100 μL
    Assay Time
    2.5 h
    Protocol
    11,12-EET/DHET ELISA Assay kit is intended for the quantitative determination of 11,12-EET/DHET in biological samples by enzyme linked immunoassay (ELISA). 11,12-EET/DHET ELISA Assay kit is for research use only and not to be used in diagnostic procedures
    Restrictions
    For Research Use only
  • Storage
    -20 °C
  • Target
    11,12-EET/DHET
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