Glutathione HPLC Assay
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- Target See all Glutathione Kits
- Glutathione
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Reactivity
- Chemical
- Application
- High Pressure Liquid Chromatography (HPLC)
- Purpose
- Most of the intracellular glutathione is reduced (approx. 90 % in EDTA-blood), only 10 % is oxidized (GSSG). The NADPH-dependent glutathione reductase maintains this steady state. Alterations in the glutathione status are involved in the pathogenesis of several diseases. In discussion are reperfusion damage, liver injury, cancer, diabetes mellitus, cataract, inflammatory diseases, chronic lymphatic edema and radiation damages. Altered glutathione concentrations might also be due to pollution, cigarette smoke, side effects of drugs and aging. In case of oxidative stress the amount of reduced glutathione is diminished. The relation of reduced to oxidized glutathione gives information about the redox and detoxification status of cells and tissue.
- Sample Type
- Blood (EDTA)
- Characteristics
- Most of the intracellular glutathione is reduced (approx. 90 % in EDTA-blood), only 10 % is oxidized (GSSG). The NADPH-dependent glutathione reductase maintains this steady state. Alterations in the glutathione status are involved in the pathogenesis of several diseases. In discussion are reperfusion damage, liver injury, cancer, diabetes mellitus, cataract, inflammatory diseases, chronic lymphatic edema and radiation damages. Altered glutathione concentrations might also be due to pollution, cigarette smoke, side effects of drugs and aging. In case of oxidative stress the amount of reduced glutathione is diminished. The relation of reduced to oxidized glutathione gives information about the redox and detoxification status of cells and tissue.
- Top Product
- Discover our top product Glutathione ELISA Kit
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Glutathione ELISA Kit
Reactivity: Various Species Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutathione Fluorescent Detection KitBCA Reactivity: Various Species, Human Fluorometric Blood, Cell Lysate, Erythrocyte Lysates, Plasma, Serum, Tissue Samples, Urine
Glutathione Colorimetric Detection KitBCA Reactivity: Various Species, Human Colorimetric Blood, Cell Lysate, Erythrocyte Lysates, Plasma, Serum, Tissue Samples, Urine
Glutathione ELISA KitReactivity: Chemical Colorimetric Competition ELISA 1.56 μg/mL - 100 μg/mL Plasma, Serum
Glutathione Assay KitBCA Blood, Cell Culture Supernatant, Cell Extracts, Plasma, Serum, Urine
SensoLyte® Glutathione Cellular Assay KitEAA Fluorometric
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- < 1 h
- Protocol
- For the determination of glutathione the sample is divided in two aliquots. One is reduced and the total amount of glutathione is measured. The other aliquot is treated without reduction solution, which determines only the reduced glutathione. During the derivatisation reaction glutathione is converted into a fluorescent probe. The following precipitation step removes high molecular substances. After centrifugation the fluorescent probe is cooled (2-8 °C) and injected into the HPLC system. The isocratic separation via HPLC at 30 °C uses a reversed phase column in two runs. One run lasts 4 minutes. The chromatograms are recorded by a fluorescence detector. The quantification is performed with the delivered EDTA-blood calibrator, the concentration is calculated by the internal standard method.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target
- Glutathione
- Abstract
- Glutathione Products
- Target Type
- Chemical
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