Mouse MDSC Flow Cocktail 1

Details for Product No. ABIN2669222, Supplier: Log in to see
Mouse (Murine)
Flow Cytometry (FACS), Isotype Control (IsoC)
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Characteristics This mouse MDSC Flow Cocktail 1 is composed of CD11b, Gr-1, and Ly-6G antibodies and corresponding isotype controls. MDSCs have been reported with CD11b and Gr-1 phenotype. MDSCs are a heterogenous population of cells that can affect T cell function. Two different subsets of MDSC exist: CD11b+Ly-6G+Ly-6Clow (granulocytic MDSC) and CD11b+ Ly-6G-Ly-6Chi (monocytic MDSC). These cells have been identified in a variety of pathological conditions including cancer and autoimmune disease. CD11b: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. Gr-1: Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation, Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity. Ly-6G: Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.
Components Mouse MDSC Flow Cocktail 1 with Isotype Ctrl, Rat IgG2a/Rat IgG2b/Rat IgG2b, Clone: M1/70/RB6-8C5/1A8 25 tests
Application Notes Optimal working dilution should be determined by the investigator.,This reagent is a multicolor cocktail of PE conjugated CD11b (clone M1/70), APC conjugated Gr-1 (clone RB6-8C5), and FITC conjugated Ly-6G (clone 1A8) at optimal concentration for three-color flow cytometric analysis.
Restrictions For Research Use only
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.
Supplier Images
Flow Cytometry (FACS) image for Mouse MDSC Flow Cocktail 1 (ABIN2669222) Mouse MDSC Flow Cocktail 1
Flow Cytometry (FACS) image for Mouse MDSC Flow Cocktail 1 (ABIN2669222) Mouse MDSC Flow Cocktail 1 (Image 2)
Background publications Dietlin, Hofman, Lund, Gilmore, Stohlman, van der Veen: "Mycobacteria-induced Gr-1+ subsets from distinct myeloid lineages have opposite effects on T cell expansion." in: Journal of leukocyte biology, Vol. 81, Issue 5, pp. 1205-12, 2007 (PubMed).

Daley, Thomay, Connolly, Reichner, Albina: "Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice." in: Journal of leukocyte biology, Vol. 83, Issue 1, pp. 64-70, 2007 (PubMed).

Coxon, Rieu, Barkalow, Askari, Sharpe, von Andrian, Arnaout, Mayadas: "A novel role for the beta 2 integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation." in: Immunity, Vol. 5, Issue 6, pp. 653-66, 1997 (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).

Stoppacciaro, Melani, Parenza, Mastracchio, Bassi, Baroni, Parmiani, Colombo: "Regression of an established tumor genetically modified to release granulocyte colony-stimulating factor requires granulocyte-T cell cooperation and T cell-produced interferon gamma." in: The Journal of experimental medicine, Vol. 178, Issue 1, pp. 151-61, 1993 (PubMed).

Fleming, Fleming, Malek: "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2399-408, 1993 (PubMed).

Brummer, Sugar, Stevens: "Immunological activation of polymorphonuclear neutrophils for fungal killing: studies with murine cells and blastomyces dermatitidis in vitro." in: Journal of leukocyte biology, Vol. 36, Issue 4, pp. 505-20, 1984 (PubMed).