Anti-Neu5Gc Antibody Kit

Details for Product No. ABIN2669225, Supplier: Log in to see
Flow Cytometry (FACS)
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Cross-Reactivity (Details) All mammals except human
Characteristics Neu5Gc (N-Glycolylneuraminic acid) is a sialic acid molecule found in most mammals but not in humans. It is derived from the most commonly expressed sialic acid, Neu5Ac, differing from it by only one oxygen atom. The gene CMAH that encodes for CMP-N-acetylneuraminic acid hydroxylase is responsible for the conversion of Neu5Ac to Neu5Gc. In humans, this gene is inactive due to a deletion mutation. As such, the human immune system sees Neu5Gc as a foreign, immunogenic molecule. Neu5Gc enters the human body primarily through dietary intake of red meat and dairy products. Neu5Gc is also incorporated, albeit to a lesser degree, into certain biopharmaceuticals, through the use of animal-derived reagents and cell lines during the manufacturing of these products. Due to its immunogenic properties, the intake and incorporation of Neu5Gc in humans causes the formation of circulating antibodies against this non-human molecule, which can lead to chronic inflammation, the exacerbation of certain diseases, and possibly reduced the efficacy of biopharmaceuticals.
Components Anti-Neu5Gc Antibody Kit, Chicken IgY, Clone: Poly21469, PURE 25 μl
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid, Lyophilized
Buffer The Neu5Gc antibody and the chicken IgY isotype control in this kit are lyophilized. The blocking solution is liquid.
Storage -20 °C
Storage Comment For long-term storage, store the lyophilized antibodies at -20°C or -70°C, and store the blocking solution between 2°C and 8°C. After reconstitution, store the antibodies between 2°C and 8°C for up to 2 weeks. For longer term storage up to 6 months, store the reconstituted product in aliquots at -20°C.
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Flow Cytometry (FACS) image for Anti-Neu5Gc Antibody Kit (ABIN2669225) Anti-Neu5Gc Antibody Kit
Flow Cytometry (FACS) image for Anti-Neu5Gc Antibody Kit (ABIN2669225) Anti-Neu5Gc Antibody Kit (Image 2)
Background publications Diaz, Padler-Karavani, Ghaderi, Hurtado-Ziola, Yu, Chen, Brinkman-Van der Linden, Varki, Varki: "Sensitive and specific detection of the non-human sialic Acid N-glycolylneuraminic acid in human tissues and biotherapeutic products." in: PLoS ONE, Vol. 4, Issue 1, pp. e4241, 2009 (PubMed).

Padler-Karavani, Yu, Cao, Chokhawala, Karp, Varki, Chen, Varki: "Diversity in specificity, abundance, and composition of anti-Neu5Gc antibodies in normal humans: potential implications for disease." in: Glycobiology, Vol. 18, Issue 10, pp. 818-30, 2008 (PubMed).

Ludwig, Bergendahl, Levenstein, Yu, Probasco, Thomson: "Feeder-independent culture of human embryonic stem cells." in: Nature methods, Vol. 3, Issue 8, pp. 637-46, 2006 (PubMed).

Bardor, Nguyen, Diaz, Varki: "Mechanism of uptake and incorporation of the non-human sialic acid N-glycolylneuraminic acid into human cells." in: The Journal of biological chemistry, Vol. 280, Issue 6, pp. 4228-37, 2005 (PubMed).

Nguyen, Tangvoranuntakul, Varki: "Effects of natural human antibodies against a nonhuman sialic acid that metabolically incorporates into activated and malignant immune cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 1, pp. 228-36, 2005 (PubMed).