PDGF-BB Homodimer ELISA Kit

Catalog No. ABIN2859308

Product Details

Target: PDGF-BB Homodimer

Binding Specificity: AA 82-190

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: For quantitative detection of human PDGF-BB in cell culture supernates, serum and plasma(heparin, EDTA).

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Natural and recombinant human PDGF-BB

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Components:

• 96-well plate precoated with anti- human PDGF-BB antibod - 1
• Lyophilized recombinant human PDGF-BB standar - 10ng/tubex2
• Biotinylated anti- human PDGF-BB antibod - 130μl(dilution 1:100)
• Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
• Sample diluent buffe - 30 ml
• Antibody diluent buffe - 12ml
• ABC diluent buffe - 12ml
• TMB color developing agen - 10ml
• TMB stop solutio - 10ml

Immunogen: Expression system for standard: E.coli
Immunogen sequence: S82-T190

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: human PDGF-BB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PDGF-BB has been precoated onto 96-well plates. Standards(E.coli, S82-T190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PDGF-BB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PDGF-BB amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PDGF-BB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PDGF-BB standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 342, Standard deviation: 18.5, CV(%): 5.4
• Sample 2: n=16, Mean(pg/ml): 818, Standard deviation: 38.5, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 1163, Standard deviation: 71, CV(%): 6.1,
• Sample 1: n=24, Mean(pg/ml): 326, Standard deviation: 25.43, CV(%): 7.8
• Sample 2: n=24, Mean(pg/ml): 794, Standard deviation: 60.4, CV(%): 7.6
• Sample 3: n=24, Mean(pg/ml): 1234, Standard deviation: 105, CV(%): 8.5

Restrictions: For Research Use only

Handling

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References

Dai, Cui, Zhang, Cheng, Liu, Yang, Fan, Wang, Lin, Zhang, Li, Mao, Wang, Su, Zhang, Peng, Yang, Hu, Yang, Huang, Xiang, Yu, Zhou, Wei, Deng: "SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin." in: Nature communications, Vol. 7, pp. 11996, (2018) (PubMed).

Liang, Wang, Zhang, Lu, Wang: "Expression and functional analysis of platelet-derived growth factor in uterine leiomyomata." in: Cancer biology & therapy, Vol. 5, Issue 1, pp. 28-33, (2006) (PubMed).

Target Details

Target: PDGF-BB Homodimer

Alternative Name: PDGF-BB

Background: Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. This gene product can exist either as a homodimer(PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide(PDGF-AB), where the dimers are connected by disulfide bonds. This gene is mapped to 22q13.1. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. This gene plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.

Gene ID: 5155

UniProt: P01127