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IGFBPI ELISA Kit

IGFBPI Reactivity: Human AA 26-259 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Pubmed (2 references)
Catalog No. ABIN411288
$369.00
Plus shipping costs $45.00
96 tests
Shipping to: United States
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  • Target See all IGFBPI ELISA Kits
    IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
    Binding Specificity
    AA 26-259
    Reactivity
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2-2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP-1
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: A26-N259
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <1pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: A26-N259
    Featured
    Discover our best selling IGFBPI ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 1 IGFBP N-terminal domain.

    Plate
    Pre-coated
    Protocol
    human IGFBP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP-1 has been precoated onto 96-well plates. Standards(NSO, A26-N259) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP-1 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IGFBP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP-1 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 145, Standard deviation: 6.09, CV(%): 4.2
    • Sample 2: n=16, Mean(pg/ml): 736, Standard deviation: 37.54, CV(%): 5.1
    • Sample 3: n=16, Mean(pg/ml): 1243, Standard deviation: 70.85, CV(%): 5.7,
    • Sample 1: n=24, Mean(pg/ml): 173, Standard deviation: 10.21, CV(%): 5.9
    • Sample 2: n=24, Mean(pg/ml): 667, Standard deviation: 42.02, CV(%): 6.3
    • Sample 3: n=24, Mean(pg/ml): 1360, Standard deviation: 102, CV(%): 7.5
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Zhang, Zhao, Hu, Liu, Chen, Chen, Du: "Distinct post-transcriptional regulation of Igfbp1 gene by hypoxia in lowland mouse and Qinghai-Tibet plateau root vole Microtus oeconomus." in: Molecular and cellular endocrinology, Vol. 376, Issue 1-2, pp. 33-42, (2013) (PubMed).

    Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." in: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).

  • Target See all IGFBPI ELISA Kits
    IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
    Alternative Name
    IGFBP1 (IGFBPI Products)
    Synonyms
    cb656, igfbp1, IGFBP-1, MGC68538, afbp, ibp1, pp12, igf-bp25, higfbp-1, IGFBP1, LOC100223208, LOC100305121, AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1, IGFBA, insulin like growth factor binding protein 1, insulin-like growth factor binding protein 1a, insulin like growth factor binding protein 1 S homeolog, insulin like growth factor binding protein 1 L homeolog, insulin-like growth factor binding protein 1, IGFBP1, igfbp1a, igfbp1.S, igfbp1.L, igfbp1, LOC100305121, Igfbp1
    Background

    Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. .

    Background: IGFBP1 is also known as amniotic fluid binding protein(AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein(IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.

    Synonyms: Insulin-like growth factor-binding protein 1,IBP-1,IGF-binding protein 1,IGFBP-1,Placental protein 12,PP12,IGFBP1,IBP1,

    Full Gene Name: Insulin-like growth factor-binding protein 1

    Cellular Localisation: Secreted.
    Gene ID
    3484
    UniProt
    P08833
    Pathways
    Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding
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