MMP2 ELISA Kit

Catalog No. ABIN411328

Human MMP2 ELISA Kit, Colorimetric assay for quantification of Human MMP2, that has been mentioned in 18+ publications.

Quick Overview for MMP2 ELISA Kit (ABIN411328)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Binding Specificity: AA 30-660

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 625 pg/mL - 40000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin)

Product Details MMP2 ELISA Kit

Minimum Detection Limit: 625 pg/mL

Purpose: Sandwich Human MMP-2 ELISA Kit to quantitate Human MMP2 in cell culture supernatants, serum and plasma (heparin).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: A30-C660

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.1%, 7.2%, 4.9%
Inter-Assay Precision (CV%): 6.1%, 8.8%, 4.9%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for MMP2 ELISA Kit (ABIN411328)

Matin, Nemati, Ghobadi, Alipanah-Moghadam, Rezagholizadeh: "The effect of conjugated linoleic acid on oxidative stress and matrix metalloproteinases 2 and 9 in patients with COPD." in: International journal of chronic obstructive pulmonary disease, Vol. 13, pp. 1449-1454, (2018) (PubMed).

Nosratzehi, Alijani, Moodi: "Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma" in: Asian Pacific journal of cancer prevention : APJCP, Vol. 18, Issue 7, pp. 1947-1951, (2017) (PubMed).

Hu, Ni, Cao, Zhang, Wu, Yin, Lang, Lu: "The Angiogenic Effect of microRNA-21 Targeting TIMP3 through the Regulation of MMP2 and MMP9." in: PLoS ONE, Vol. 11, Issue 2, pp. e0149537, (2016) (PubMed).

Bi, Zeng, Zhao, Wei, Yu, Wang, Yu, Cao, Shan, Wei: "miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα." in: Molecular therapy. Nucleic acids, Vol. 5, Issue 9, pp. e368, (2016) (PubMed).

Simmers, Gishto, Vyavahare, Kothapalli: "Nitric oxide stimulates matrix synthesis and deposition by adult human aortic smooth muscle cells within three-dimensional cocultures." in: Tissue engineering. Part A, Vol. 21, Issue 7-8, pp. 1455-70, (2015) (PubMed).

Liu, Zou, Zhou, Gong, Wang, Cai, Tan, Fang: "miR-155 Regulates Glioma Cells Invasion and Chemosensitivity by p38 Isforms In Vitro." in: Journal of cellular biochemistry, Vol. 116, Issue 7, pp. 1213-21, (2015) (PubMed).

Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

Qin, Liu, Li, Liu, Li, Gao, Shao, Zhen: "MMP-2/9-oriented combinations enhance antitumor efficacy of EGFR/HER2-targeting fusion proteins and gemcitabine." in: Oncology reports, Vol. 32, Issue 1, pp. 121-30, (2014) (PubMed).

Yuan, Yang, Zhou, Gao, Qiu, Fang, Ding, Xiao et al.: "Knockdown of sphingosine kinase 1 inhibits the migration and invasion of human rheumatoid arthritis fibroblast-like synoviocytes by down-regulating the PI3K/AKT activation and MMP-2/9 production in ..." in: Molecular biology reports, Vol. 41, Issue 8, pp. 5157-65, (2014) (PubMed).

Mazani, Fard, Baghi, Nemati, Mogadam: "Effect of pomegranate juice supplementation on matrix metalloproteinases 2 and 9 following exhaustive exercise in young healthy males." in: JPMA. The Journal of the Pakistan Medical Association, Vol. 64, Issue 7, pp. 785-90, (2014) (PubMed).

Hopps, Lo Presti, Montana, Canino, Averna, Caimi: "Study of the correlations among some parameters of the oxidative status, gelatinases, and their inhibitors in a group of subjects with metabolic syndrome." in: Mediators of inflammation, Vol. 2014, pp. 510619, (2014) (PubMed).

Xu, Ling, Zhu, Fan, Zhang: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." in: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).

Tang, Tang, Fang, Liang, Zhang: "Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells." in: World journal of gastroenterology, Vol. 19, Issue 10, pp. 1582-92, (2013) (PubMed).

Hopps, Lo Presti, Montana, Noto, Averna, Caimi: "Gelatinases and their tissue inhibitors in a group of subjects with metabolic syndrome." in: Journal of investigative medicine : the official publication of the American Federation for Clinical Research, Vol. 61, Issue 6, pp. 978-83, (2013) (PubMed).

Jiang, Huang, Yu, Wu, Zhou, Li: "Overexpression of HTRA1 leads to down-regulation of fibronectin and functional changes in RF/6A cells and HUVECs." in: PLoS ONE, Vol. 7, Issue 10, pp. e46115, (2013) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

Yu, Zhu, Mi, Chen, Pan, Wei: "Anti-angiogenic genistein inhibits VEGF-induced endothelial cell activation by decreasing PTK activity and MAPK activation." in: Medical oncology (Northwood, London, England), Vol. 29, Issue 1, pp. 349-57, (2012) (PubMed).

Pan, Ren, Ma, Liu, Yu, Ji, Pan, Li, Yang, Lv, Shen, Chen, Zhang, Willard, He, Zheng: "High-density lipoprotein of patients with type 2 diabetes mellitus elevates the capability of promoting migration and invasion of breast cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 131, Issue 1, pp. 70-82, (2012) (PubMed).

Target Details for MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: MMP2

Background:

Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant human MMP-2, consisting of 631 amino acids with the molecular mass of 71KDa. The detected MMP-2 includes zymogen and active enzyme.

Gene Full Name: matrix metallopeptidase 2

Gene ID: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response