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MMP2 ELISA Kit (Matrix Metalloproteinase 2) ELISA Kit

MMP2 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pubmed (25)
Catalog No. ABIN6730864
$461.05
Plus shipping costs $45.00
96 tests
local_shipping Shipping to: United States
Delivery in 9 to 11 Business Days
  • Target
    MMP2
    Reactivity
    • 9
    • 9
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.78 ng/mL - 50 ng/mL
    Minimum Detection Limit
    0.78 ng/mL
    Application
    ELISA
    Purpose
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP2 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

    We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 2 (MMP2)
    Cross-Reactivity (Details)
    No significant cross-reactivity or interference between Matrix Metalloproteinase 2 (MMP2) and analogues was observed.
    Sensitivity
    0.27 ng/mL
    Components
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
  • Comment

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Sample Volume
    100 μL
    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards,
    2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
    3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
    4. Aspirate and wash 3 times,
    5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    6. Aspirate and wash 5 times,
    7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    8. Add 50μL Stop Solution. Read at 450nm immediately.
    Reagent Preparation
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 100 ng/mL. Firstly dilute the stock solution to 50 ng/mL and the diluted standard serves as the highest standard (50 ng/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    6. Contaminated water or container for reagent preparation will influence the detection result.
    Sample Preparation
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV < 10%
    Inter-Assay: CV < 12%
    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Storage
    4 °C/-20 °C
    Storage Comment
    1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
    2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.
    .
    Expiry Date
    6 months
  • Bai, Wang, Wang, Li, Zhao, Wu, Yang, Deng, Zhang: "The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 44, Issue 5, pp. 1882-1895, 2018 (PubMed).

    Wang, Jiang, An, Mao, Lin, Sun: "Effect of a synthetic inhibitor of urokinase plasminogen activator on the migration and invasion of human cervical cancer cells in vitro." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 4273-4280, 2018 (PubMed).

    Zhu, Li, Li, Jiang: "MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1." in: Human cell, Vol. 30, Issue 4, pp. 290-299, 2018 (PubMed).

    Yan, Zhu, Zhang, Li, Li, Wang, Leng: "HMGB1-RAGE signaling pathway in pPROM." in: Taiwanese journal of obstetrics & gynecology, Vol. 57, Issue 2, pp. 211-216, 2018 (PubMed).

    Liu, Qu, Zhou, Huang, Jia, Huang, Qian, Xia, Yu: "Syntenin-targeted peptide blocker inhibits progression of cancer cells." in: European journal of medicinal chemistry, Vol. 154, pp. 354-366, 2018 (PubMed).

    Prestigiacomo, Weston, Messner, Lampart, Suter-Dick: "Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis." in: PLoS ONE, Vol. 12, Issue 6, pp. e0179995, 2017 (PubMed).

    Isik, Gursul, Peker, Aydın, Fırat, Yılmaz: "Metalloproteinases and Their Inhibitors in Patients with Inguinal Hernia." in: World journal of surgery, Vol. 41, Issue 5, pp. 1259-1266, 2017 (PubMed).

    Ji, Zhang, Zhao: "Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activity." in: Human cell, Vol. 30, Issue 2, pp. 124-132, 2017 (PubMed).

    Ying, Jiang, He, Yu, Chen, Chen, Ru, Chen, Chen, Zhu, Li, Zhang, Guo, Yin, Zhang, Lou: "Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases." in: Disease markers, Vol. 2017, pp. 5756102, 2017 (PubMed).

    Huang, Liu, Huang, Li, Gan, Shen: "1,25-Dihydroxyvitamin D3 alleviates salivary adenoid cystic carcinoma progression by suppressing GPX1 expression through the NF-κB pathway." in: International journal of oncology, Vol. 48, Issue 3, pp. 1271-9, 2016 (PubMed).

    Li, Sun, Liu: "MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 38, Issue 5, pp. 1915-27, 2016 (PubMed).

    Serra, Gallelli, Butrico, Buffone, Caliò, De Caridi, Massara, Barbetta, Amato, Labonia, Mimmi, Iaccino, de Franciscis: "From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases." in: International wound journal, 2016 (PubMed).

    Zhou, Han, Zhang, Shi, Zhou, Liu, Jia: "MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1." in: Human cell, Vol. 30, Issue 1, pp. 30-40, 2016 (PubMed).

    Ding, Zhang, Huang, Liu, Lu, Sun: "Cadherin-11 involves in synovitis and increases the migratory and invasive capacity of fibroblast-like synoviocytes of osteoarthritis." in: International immunopharmacology, Vol. 26, Issue 1, pp. 153-61, 2015 (PubMed).

    Wei, Tao, Zhang, Han, Zhu, Miao, Li, Qiao: "Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 4481-91, 2015 (PubMed).

    Zhu, Fan, Abdel-Halim, Zhang, Zhu: "Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V(2+) conjugates." in: Biosensors & bioelectronics, Vol. 77, pp. 339-46, 2015 (PubMed).

    Yu, Wang, Tang, Zhang, Shang: "RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-?B signaling pathway in trophoblast cells." in: Acta histochemica, Vol. 116, Issue 5, pp. 911-9, 2014 (PubMed).

    Yang, Zhang, Gong, Liu, Bai, Xu, Zhou: "Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion." in: Journal of perinatology : official journal of the California Perinatal Association, Vol. 35, Issue 1, pp. 16-22, 2014 (PubMed).

    Jiang, Zhang, Teng, Zhang, Zhang, Huang: "Downregulation of CD9 in keratinocyte contributes to cell migration via upregulation of matrix metalloproteinase-9." in: PLoS ONE, Vol. 8, Issue 10, pp. e77806, 2013 (PubMed).

    Barreto, Coester, Struijk, Krediet: "Can effluent matrix metalloproteinase 2 and plasminogen activator inhibitor 1 be used as biomarkers of peritoneal membrane alterations in peritoneal dialysis patients?" in: Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis, Vol. 33, Issue 5, pp. 529-37, 2013 (PubMed).

  • Target
    MMP2
    Abstract
    MMP2 ELISA Kit Abstract
    Synonyms
    2-MMP, CG1794, DM2-MMP, Dm2-MMP, Dmel\\CG1794, MMP2, anon-WO0118547.84, dm-2MMP, dmmp2, l(2)02353, mmp2, wu:fa99h12, wu:fk89d01, fgmmp-2, Mmp-2, LOC100135793, Clg4a, GelA, MMP-2, CLG4, CLG4A, MMP-II, MONA, TBE-1, matrix metallopeptidase 2, Matrix metalloproteinase 2, matrix metalloproteinase 2, matrix metalloproteinase-2, MMP2, Mmp2, mmp2, LOC657982, LOC100135793
    UniProt
    P08253
    Pathways
    Activation of Innate immune Response
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