SERPINA1 ELISA Kit

Catalog No. ABIN454184

Product Details SERPINA1 ELISA Kit

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12-200 ng/mL

Minimum Detection Limit: 3.12 ng/mL

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of human ? 1-Antitrypsin, ? 1-AT concentrations in cell culture supernates, serum, plasma and other biological fluids.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural human ? 1-AT.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 9.75 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Homo sapiens,Human,Alpha-1-antitrypsin,Alpha-1 protease inhibitor,Alpha-1-antiproteinase,Serpin A1,SERPINA1,AAT,PI,PRO0684,PRO2209

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to α 1-AT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for α 1-AT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain α 1-AT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a 2 sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of α 1-AT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for SERPINA1

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Alternative Name: SERPINA1 (SERPINA1 Products)

Background: Alpha 1-Antitrypsin or α 1-antitrypsin (A1AT) is a glycoprotein and generally known as serum trypsin inhibitor. The correct name, however, is alpha-1 proteinase inhibitor (A1PI) because it is a serine protease inhibitor (serpin) inhibiting a wide variety of proteases. It protects tissues from enzymes of inflammatory cells, especially elastase, and is present in human blood at 1.5 - 3.5 gram/liter, but the concentration can rise manyfolds upon acute inflammation. In its absence, elastase is free to break down elastin -- which contributes to the elasticity of the lungs -- resulting in respiratory complications such as emphysema leading finally to COPD (chronic obstructive pulmonary disease). A1AT is a 52 kDa serpin (serine protease inhibitor), and in medicine it is considered the most prominent serpin, given the fact that the words α 1-antitrypsin and protease inhibitor (Pi) are often used interchangeably. Most serpins inactivate enzymes by binding to them covalently, requiring very high levels to perform their function. In the acute phase reaction, a further elevation is required to "limit" the damage caused by activated neutrophil granulocytes and their enzyme elastase, which breaks down the connective tissue fiber elastin. Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins which can polymerise and accumulate in the liver (infantile hepatic cirrhosis).