ICAM1 ELISA Kit

Catalog No. ABIN455472

Product Details ICAM1 ELISA Kit

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of mouse intercellular adhesion molecule 1,ICAM-1 concentrations in cell culture supernates, serum, plasma and other biological fluids.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse ICAM-1/CD54.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 0.078 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Mus musculus,Mouse,Intercellular adhesion molecule 1,ICAM-1,MALA-2,MyD10,Icam1,Icam-1,CD54

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICAM-1/CD54. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ICAM-1/CD54 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ICAM-1/CD54, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ICAM-1/CD54 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: Icam1 (ICAM1 Products)

Target Type: Viral Protein

Background: Adhesion molecules mediate the interaction of cells with the extracellular matrix and with other cells. The immunoglobulin superfamily of proteins contains a large class of adhesion molecules with multiple immunoglobulin-like domains. ICAM is a member of this family. It is a 90 kDa type-I transmembrane glycoprotein with five Ig-like extracellular domains. The most important ligands for ICAM-1 are the _2 integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18), which are expressed on leukocytes. ICAM-1 thus mediates the adhesion of leukocytes to ICAM-1-expressing cells. ICAM-1 also binds fibrinogen, hyaluronan, Rhinoviruses, Plasmodium falciparum-infected erythrocytes and CD43 (sialophorin) ICAM-1 is either a transmembrane protein (mICAM-1) or soluble (sICAM-1). mICAM-1 is expressed on endothelial and epithelial cells, lymphocytes, monocytes, eosinophils, keratinocytes, dendritic cells, hematopoietic stem cells, hepatocytes and fibroblasts. Regulation of ICAM-1 expression is cell specific. Up-regulation generally is by inflammatory cytokines (TNF- α , IFN- γ and IL-1) and down-regulation generally is by anti-inflammatory agents (e.g.glucocorticoids). One important, well-characterized function of ICAM-1 is immune-cell trafficking. At sites of inflammation, inflammatory cytokines induce up-regulation of ICAM-1 expression on vascular endothelial cells and activation of leukocyte integrins (LFA-1 and Mac-1). This leads to adhesion of leukocytes to the local endothelium, an essential step in migration of leukocytes to the site of inflammation. sICAM-1 has been reported in serum, cerebrospinal fluid and bronchoalveolar lavage. sICAM-1 likely arises by proteolytic cleavage of mICAM-1, synthesis from an alternatively spliced message has not been found. In general, elevated levels of serum ICAM-1 appear to be associated with inflammatory conditions and certain malignancies. It has, however, been pointed out that in inflammatory conditions, where the ligands LFA-1 and Mac-1 are likely to be activated, 2 binding and clearance of sICAM-1 might be enhanced, so that a reciprocal relationship between sICAM-1 levels and inflammation also is possible.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling