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WAP, Follistatin/kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 2 (WFIKKN2) ELISA Kit ELISA Kit

WFIKKN2 Reactivity: Human Colorimetric Sandwich ELISA 20-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4883008
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  • Target
    WAP, Follistatin/kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 2 (WFIKKN2)
    • 2
    • 1
    • 1
    Detection Method
    Method Type
    Sandwich ELISA
    Detection Range
    20-5000 pg/mL
    Minimum Detection Limit
    20 pg/mL
    Human GASP-1 (WFIKKNRP) ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE-2, aFGF, Albumin, AMICA, Angiopoietin-4, BAFF, CA19-9, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, Decorin, Dkk-3, DLL1, Fetuin A, FOLR1, Furin, GASP-2, GCSF R, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RANK, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRAIL R2, TRANCE, Troponin I, uPA, VE-Cadherin, WISP-1.
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Recommended Dilution for serum and plasma samples10 fold
    Sample Volume
    100 μL
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target
    WAP, Follistatin/kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 2 (WFIKKN2)
    Alternative Name
    GASP-1///WFIKKNRP (WFIKKN2 ELISA Kit Abstract)
    GASP-1, WFDC20B, WFIKKNRP, hGASP-1, 2610304F08Rik, AY100450, Gasp1, WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2, WFIKKN2, Wfikkn2
    Gene ID
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