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SPI1 ELISA Kit

SPI1 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4884304
  • Target See all SPI1 ELISA Kits
    SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
    Reactivity
    • 4
    • 3
    • 3
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human PU.1/Spi-1 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes .
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product SPI1 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all SPI1 ELISA Kits
    SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
    Alternative Name
    PU.1 (SPI1 Products)
    Synonyms
    OF ELISA Kit, PU.1 ELISA Kit, SFPI1 ELISA Kit, SPI-1 ELISA Kit, SPI-A ELISA Kit, Dis-1 ELISA Kit, Dis1 ELISA Kit, Sfpi-1 ELISA Kit, Sfpi1 ELISA Kit, Spi-1 ELISA Kit, Tcfpu1 ELISA Kit, Tfpu.1 ELISA Kit, Pu.1 ELISA Kit, cb1086 ELISA Kit, pu.1 ELISA Kit, pu1 ELISA Kit, sfpi1 ELISA Kit, si:by184l24.2 ELISA Kit, spi1 ELISA Kit, SPI1/PU.1 ELISA Kit, spi1l ELISA Kit, zgc:152991 ELISA Kit, Spi-1 proto-oncogene ELISA Kit, spleen focus forming virus (SFFV) proviral integration oncogene ELISA Kit, Spi-1 proto-oncogene b ELISA Kit, Spi-1 proto-oncogene a ELISA Kit, SPI1 ELISA Kit, Spi1 ELISA Kit, spi1b ELISA Kit, spi1a ELISA Kit
    Gene ID
    6688
    Pathways
    Stem Cell Maintenance
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