CD40 ELISA Kit (CD40 Molecule, TNF Receptor Superfamily Member 5)

Details for Product CD40 ELISA Kit No. ABIN4889785, Supplier: Log in to see
Antigen
  • Bp50
  • CDW40
  • TNFRSF5
  • p50
  • AI326936
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • TNFSF5
  • CD40 molecule
  • CD40 antigen
  • CD40
  • Cd40
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
29
28
6
2
2
2
1
1
1
Detection Method
Colorimetric
Method Type
DNA-Binding ELISA
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Human NF-κB p50 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical NF-κB binding sequences to semi-quantitatively detect active NF-κB in lysates or nuclear extracts. Only available in North America.
Sample Type Cell Lysate, Nuclear Extract
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity The olionucleotide/antibody pair provided in this kit recognizes human NF-κB p50 in whole lysates and nuclear extracts.
Characteristics
  • Specific transcription factor-DNA binding assay
  • Perfect alternative to EMSA
  • Easy to perform in an ELISA format
  • Non-radioactive assay
  • High throughput (96 well plate format)
  • Assay can be completed within 5 hours
Components
  • 96-well Strip Microplate pre-coated with DNA probes
  • DNA Binding Buffer
  • Positive Control Sample
  • Specific Competitor DNA probe
  • Non-specific Competitor DNA probe
  • Assay Reagent
  • DTT
  • Wash Buffer
  • Primary Antibody
  • HRP-conjugated Secondary Antibody
  • TMB One-Step Substrate Reagent
  • Stop Solution
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions Absorbent paper
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Plasmids, Primers & others Plasmids, Primers & others CD40 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name p50 (CD40 ELISA Kit Abstract)
UniProt P19838
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared HRP-secondary antibody to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Activity Assay (AcA) image for CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) ELISA Kit (ABIN4889785) Transcription factor activity assay of NF-κB p50 from nuclear extracts of HeLa cells ...
Activity Assay (AcA) image for CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) ELISA Kit (ABIN4889785) Transcription factor activity assay of NF-κB p50 from nuclear extracts of HeLa cells ...