Thrombomodulin ELISA Kit

Catalog No. ABIN5510595

Product Details Thrombomodulin ELISA Kit

Target: Thrombomodulin (THBD)

Binding Specificity: AA 19-515

Reactivity: Monkey

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate Thrombomodulin

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: A19-S515

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Characteristics:

Sequence similarities: Contains 1 C-type lectin domain.

Tissue Specificity: Endothelial cells are unique in synthesizing thrombomodulin.

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: THBD (THBD Products)

Background:

Protein Function: Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

Background: Thrombomodulin(TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on monkey primate mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74 kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor(TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene(THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome(aHUS).

Synonyms: Thrombomodulin,TM,Fetomodulin,CD141,THBD,THRM,

Full Gene Name: Thrombomodulin

Cellular Localisation: Membrane, Single-pass type I membrane protein.

UniProt: P07204