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NFYA ELISA Kit (Nuclear Transcription Factor Y, alpha) ELISA Kit

NFYA Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate
Catalog No. ABIN5526700
$480.70
Plus shipping costs $45.00
96 tests
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  • Target
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    DNA-Binding ELISA
    Application
    ELISA
    Purpose
    Human NF-YA Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical NF-YA binding sequences to semi-quantitatively detect active NF-YA in lysates or nuclear extracts. Only available in North America.
    Sample Type
    Cell Lysate, Nuclear Extract
    Analytical Method
    Semi-Quantitative
    Specificity
    The olionucleotide/antibody pair provided in this kit recognizes mouse NFYA in whole lysates and nuclear extracts.
    Characteristics
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    Components
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Expiry Date
    6 months
  • Target
    Alternative Name
    NF-YA (NFYA ELISA Kit Abstract)
    Synonyms
    wu:fb16e03, ik:tdsubc_2b6, xx:tdsubc_2b6, NFYA, CBF-A, CBF-B, HAP2, NF-YA, AA407810, Cbf-b, SEZ-10, SEZ10, nuclear transcription factor Y, alpha, nuclear transcription factor Y subunit alpha, nuclear transcription factor-Y alpha, nfya, NFYA, Nfya
    Gene ID
    4800
    UniProt
    P23511
    Pathways
    Regulation of Lipid Metabolism by PPARalpha
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