CYP11B2 ELISA Kit

Catalog No. ABIN5674859

Product Details CYP11B2 ELISA Kit

Target: CYP11B2 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 2 (CYP11B2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0117 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for CYP11B2

Target: CYP11B2 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 2 (CYP11B2))

Alternative Name: Aldosterone Synthase (ALDOS) (CYP11B2 Products)

Synonyms: ALDOS ELISA Kit, CPN2 ELISA Kit, CYP11B ELISA Kit, CYP11BL ELISA Kit, CYPXIB2 ELISA Kit, P-450C18 ELISA Kit, P450C18 ELISA Kit, P450aldo ELISA Kit, Cpn2 ELISA Kit, Cyp11b ELISA Kit, Cyp11b-2 ELISA Kit, Cp45as ELISA Kit, Cyp11b3 ELISA Kit, RNCP45AS ELISA Kit, cytochrome P450 family 11 subfamily B member 2 ELISA Kit, cytochrome P450, family 11, subfamily b, polypeptide 2 ELISA Kit, aldosterone synthase ELISA Kit, cytochrome P450, family 11, subfamily B, polypeptide 2 ELISA Kit, cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 2 ELISA Kit, CYP11B2 ELISA Kit, Cyp11b2 ELISA Kit

Background: Alternative name: CPN2, CYP11B, CYP11BL, CYP11B2, P450C18, P450aldo, Steroid 18-hydroxylase, Aldosterone-synthesizing enzyme, Cytochrome P450 Family 11 Subfamily B Polypeptide 2

Gene ID: 11677

UniProt: P45376

Pathways: ACE Inhibitor Pathway, Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, C21-Steroid Hormone Metabolic Process, Feeding Behaviour