ACAT1 ELISA Kit (Acetyl-CoA Acetyltransferase 1)

Details for Product ACAT1 ELISA Kit No. ABIN5674860, Supplier: Log in to see
Antigen
  • ACAT
  • MAT
  • T2
  • THIL
  • RATACAL
  • 6330585C21Rik
  • Acat
  • fd16h07
  • fd20g06
  • wu:fd16h07
  • wu:fd20g06
  • zgc:86832
  • acat1-a
  • acetyl-CoA acetyltransferase 1
  • acetyl-Coenzyme A acetyltransferase 1
  • acetyl-CoA acetyltransferase 1 L homeolog
  • ACAT1
  • Acat1
  • acat1
  • acat1.L
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
8
7
3
1
Method Type
Sandwich ELISA
Detection Range
15.625 pg/mL - 1000 pg/mL
Minimum Detection Limit
15.625 pg/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Sample Type Cell Lysate, Tissue Homogenate
Analytical Method Quantitative
Detection Method Colorimetric
Sensitivity 4.75 pg/mL
Components Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual
Material not included 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
Plasmids, Primers & others Plasmids, Primers & others ACAT1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) (ACAT1 ELISA Kit Abstract)
Background Alternative name: THIL, ACAT, T2, MAT, Acetoacetyl Coenzyme A Thiolase
Gene ID 38
UniProt P24752
Application Notes Optimal working dilution should be determined by the investigator.
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.
Restrictions For Research Use only
Storage 4 °C
Expiry Date 12 months
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