ADH7 ELISA Kit

Catalog No. ABIN5674863

Product Details ADH7 ELISA Kit

Target: ADH7 (Alcohol Dehydrogenase 7 (Class IV), mu Or sigma Polypeptide (ADH7))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0058 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ADH7

Target: ADH7 (Alcohol Dehydrogenase 7 (Class IV), mu Or sigma Polypeptide (ADH7))

Alternative Name: Alcohol Dehydrogenase 7 (ADH7) (ADH7 Products)

Synonyms: ADH4 ELISA Kit, AI325182 ELISA Kit, Adh-3 ELISA Kit, Adh-3e ELISA Kit, Adh-3t ELISA Kit, Adh3 ELISA Kit, Adh3-e ELISA Kit, Adh3-t ELISA Kit, Adh4 ELISA Kit, Adt-1 ELISA Kit, ADH7 ELISA Kit, adh6 ELISA Kit, adh7 ELISA Kit, adh7.L ELISA Kit, alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide ELISA Kit, uncharacterized LOC100232857 ELISA Kit, alcohol dehydrogenase class 4 mu/sigma chain ELISA Kit, alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide S homeolog ELISA Kit, ADH7 ELISA Kit, Adh7 ELISA Kit, LOC100232857 ELISA Kit, LOC100512795 ELISA Kit, adh7.S ELISA Kit

Background: Alternative name: Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase

Gene ID: 171178

UniProt: P41682