Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB) ELISA Kit

Details for Product No. ABIN5674883
Kits with alternative reactivity to:
Detection Method
Method Type
Sandwich ELISA
Detection Range
0.156 ng/mL - 10 ng/mL
Minimum Detection Limit
0.156 ng/mL
Sample Type Tissue Homogenate
Analytical Method Quantitative
Detection Method Colorimetric
Sensitivity 0067 ng/mL
Components Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual
Material not included 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
Alternative Name Succinate Dehydrogenase Complex Subunit B (SDHB) (SDHB ELISA Kit Abstract)
Background Alternative name: SDH1, SDH, Ip, Iron Sulfur, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Gene ID 6390
UniProt P21912
Application Notes Optimal working dilution should be determined by the investigator.
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.
Restrictions For Research Use only
Storage 4 °C
Expiry Date 12 months