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SDHB ELISA Kit (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip)) ELISA Kit

SDHB Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Catalog No. ABIN5674883
$908.41
Plus shipping costs $45.00
96 tests
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  • Target
    SDHB
    Reactivity
    Human
    • 1
    • 1
    • 1
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0067 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target
    SDHB
    Alternative Name
    Succinate Dehydrogenase Complex Subunit B (SDHB) (SDHB ELISA Kit Abstract)
    Synonyms
    succinate dehydrogenase 2-3, CG3283, Dmel\\CG3283, Ip, SDH, SDH-IP, SDH-Ip, SDHb, sdhB, CWS2, IP, PGL4, SDH1, SDH2, SDHIP, 0710008N11Rik, fj46d06, wu:fj46d06, succinate dehydrogenase complex iron sulfur subunit B, succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, Succinate dehydrogenase, subunit B (iron-sulfur), succinate dehydrogenase complex subunit B, iron sulfur (Ip), succinate dehydrogenase complex, subunit B, iron sulfur (Ip), succinate dehydrogenase complex subunit B, iron sulfur (Ip) S homeolog, SDHB, SDH2-3, SdhB, sdhb, Sdhb, sdhb.S
    Background
    Alternative name: SDH1, SDH, Ip, Iron Sulfur, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
    Gene ID
    6390
    UniProt
    P21912
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