SDHB ELISA Kit

Catalog No. ABIN5674883

Product Details SDHB ELISA Kit

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0067 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for SDHB

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Alternative Name: Succinate Dehydrogenase Complex Subunit B (SDHB) (SDHB Products)

Synonyms: succinate dehydrogenase 2-3 ELISA Kit, CG3283 ELISA Kit, Dmel\\CG3283 ELISA Kit, Ip ELISA Kit, SDH ELISA Kit, SDH-IP ELISA Kit, SDH-Ip ELISA Kit, SDHb ELISA Kit, sdhB ELISA Kit, CWS2 ELISA Kit, IP ELISA Kit, PGL4 ELISA Kit, SDH1 ELISA Kit, SDH2 ELISA Kit, SDHIP ELISA Kit, 0710008N11Rik ELISA Kit, fj46d06 ELISA Kit, wu:fj46d06 ELISA Kit, succinate dehydrogenase complex iron sulfur subunit B ELISA Kit, succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3 ELISA Kit, Succinate dehydrogenase, subunit B (iron-sulfur) ELISA Kit, succinate dehydrogenase complex subunit B, iron sulfur (Ip) ELISA Kit, succinate dehydrogenase complex, subunit B, iron sulfur (Ip) ELISA Kit, succinate dehydrogenase complex subunit B, iron sulfur (Ip) S homeolog ELISA Kit, SDHB ELISA Kit, SDH2-3 ELISA Kit, SdhB ELISA Kit, sdhb ELISA Kit, Sdhb ELISA Kit, sdhb.S ELISA Kit

Background: Alternative name: SDH1, SDH, Ip, Iron Sulfur, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

Gene ID: 6390

UniProt: P21912