TNFRSF1A ELISA Kit (Tumor Necrosis Factor Receptor Superfamily, Member 1A)

Details for Product TNFRSF1A ELISA Kit No. ABIN625089, Supplier: Log in to see
Antigen
  • CD120a
  • FPF
  • MS5
  • TBP1
  • TNF-R
  • TNF-R-I
  • TNF-R55
  • TNFAR
  • TNFR1
  • TNFR1-d2
  • TNFR55
  • TNFR60
  • p55
  • p55-R
  • p60
  • TNF-R1
  • TNF-alphaR1
  • TNFRI
  • TNFRp55
  • TNFalpha-R1
  • Tnfr-2
  • Tnfr1
  • fpf
  • tbp1
  • p55-r
  • tnf-r
  • tnfar
  • tnfr1
  • cd120a
  • tnfr55
  • tnfr60
  • xtnfr1
  • wu:fj65b10
  • zgc:85914
  • TNFR I
  • TNFRSF1A
  • TNF receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1a
  • TNF receptor superfamily member 1A S homeolog
  • TNFRSF1A
  • Tnfrsf1a
  • tnfrsf1a.S
  • tnfrsf1a
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
21
15
12
5
4
3
2
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
1-400 pg/mL
Minimum Detection Limit
1 pg/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Human TNF RI (TNFRSF1A) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity 1 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Alternative Name TNF RI (TNFRSF1A ELISA Kit Abstract)
Background Gene Names: TNFRSF1A TNFAR TNFR1
Protein names: Tumor necrosis factor receptor superfamily member 1A (Tumor necrosis factor receptor 1) (TNF-R1) (Tumor necrosis factor receptor type I) (TNF-RI) (TNFR-I) (p55) (p60) (CD antigen CD120a) [Cleaved into: Tumor necrosis factor receptor superfamily member 1A, membrane form, Tumor necrosis factor-binding protein 1 (TBPI)]
Gene ID 7132
UniProt P19438
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C
Application Notes Recommended Dilution for serum and plasma samples20 - 200 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
Supplier Images
ELISA image for Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA Kit (ABIN625089) Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA Kit
Product cited in: Fernández-Juárez, Villacorta Perez, Luño Fernández, Martinez-Martinez, Cachofeiro, Barrio Lucia, Tato Ribera, Mendez Abreu, Cordon, Oliva Dominguez, Praga Terente: "High levels of circulating TNFR1 increase the risk of all-cause mortality and progression of renal disease in type 2 diabetic nephropathy." in: Nephrology (Carlton, Vic.), Vol. 22, Issue 5, pp. 354-360, 2017 (PubMed). (Sample species: Human). Further details: Sample: Serum (Type 2 Diabetes patients with renal diseases)

Gao, Camous, Lu, Lim, Larbi, Ng: "Novel inflammatory markers associated with cognitive performance: Singapore Longitudinal Ageing Studies." in: Neurobiology of aging, Vol. 39, pp. 140-6, 2016 (PubMed). (Sample species: Human). Further details: Sample: Plasma (Cognitive Performance)

Wu, Ding, Han, Arriens, Wei, Han, Pedroza, Jiang, Anolik, Petri, Sanz, Saxena, Mohan: "Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study." in: Journal of proteome research, Vol. 15, Issue 7, pp. 2102-14, 2016 (PubMed). (Sample species: Human). Further details: Sample: Serum (Lupus Nephritis patients)

Sennikov, Alshevskaya, Shkaruba, Chumasova, Sizikov, Lopatnikova: "Expression of TNF? membrane-bound receptors in the peripheral blood mononuclear cells (PMBC) in rheumatoid arthritis patients." in: Cytokine, Vol. 73, Issue 2, pp. 288-94, 2015 (PubMed).

Cuchacovich, Hagan, Khan, Richert, Espinoza: "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." in: Clinical rheumatology, Vol. 30, Issue 1, pp. 133-7, 2011 (PubMed).

Issersohn: "[Radiologic examination of the colon with double contrast medium according to the Welin method]." in: Dapim refuiim. Folia medica, Vol. 24, Issue 1, pp. 27-31, 1970 (PubMed).

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