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IL10RB ELISA Kit
IL10RB
Reactivity: Human
Colorimetric
Sandwich ELISA
5-3000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details IL10RB ELISA Kit
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Target
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IL10RB
(Interleukin 10 Receptor, beta (IL10RB))
Reactivity
All reactivities for IL10RB elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
5-3000 pg/mL
Minimum Detection Limit
5 pg/mL
Application
ELISA
Purpose
Human IL-10 R beta ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity
5 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for IL10RB
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Target
See all IL10RB ELISA Kits
IL10RB
(Interleukin 10 Receptor, beta (IL10RB))
Alternative Name
IL-10 R beta (IL10RB Products )
Synonyms
IL10R2 ELISA Kit, IL10RB ELISA Kit, il10r2 ELISA Kit, CDW210B ELISA Kit, CRF2-4 ELISA Kit, CRFB4 ELISA Kit, D21S58 ELISA Kit, D21S66 ELISA Kit, IL-10R2 ELISA Kit, 6620401D04Rik ELISA Kit, AI528744 ELISA Kit, Crfb4 ELISA Kit, D16H21S58 ELISA Kit, D21S58h ELISA Kit, Il10r2 ELISA Kit, RGD1560373 ELISA Kit, interleukin 10 receptor subunit beta ELISA Kit, interleukin 10 receptor subunit beta S homeolog ELISA Kit, interleukin-10 receptor subunit beta ELISA Kit, interleukin 10 receptor, beta ELISA Kit, IL10RB ELISA Kit, il10rb.S ELISA Kit, LOC473966 ELISA Kit, LOC478404 ELISA Kit, il10rb ELISA Kit, Il10rb ELISA Kit
Background
Gene Names: IL10RB CRFB4 D21S58 D21S66 Protein names: Interleukin-10 receptor subunit beta (IL-10 receptor subunit beta) (IL-10R subunit beta) (IL-10RB) (Cytokine receptor class-II member 4) (Cytokine receptor family 2 member 4) (CRF2-4) (Interleukin-10 receptor subunit 2) (IL-10R subunit 2) (IL-10R2) (CD antigen CDw210b)
Gene ID
3588
UniProt
Q08334
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