BAFF ELISA Kit

Catalog No. ABIN6719675

Product Details BAFF ELISA Kit

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.9 pg/mL - 3000 pg/mL

Minimum Detection Limit: 46.9 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: B lymphocyte stimulator, B-cell-activating factor, BAFF, BLyS, CD257, Dendritic cell-derived TNF-like molecule, soluble form, TALL-1, TN13B_HUMAN, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, Tumor necrosis factor ligand superfamily member 13b

Background: B-cell activating factor(BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers(i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF(A127-L309) with molecular weight 23.2KDa.

Gene Name: TNFSF13B

Production: Natural and recombinant mouse BAFF. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A127-L309

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 188pg/mL, 94pg/mL, 46.9pg/mL mouse BAFF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse BAFF standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 46.9pg/mL-3000pg/mL

Other Performance Data: Expiration Date: 2-8° C - 06/10/2018 -20° C - 12/11/2018

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for BAFF

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: BAFF (TNFSF13B Products)

Synonyms: BAFF ELISA Kit, BLYS ELISA Kit, CD257 ELISA Kit, DTL ELISA Kit, TALL-1 ELISA Kit, TALL1 ELISA Kit, THANK ELISA Kit, TNFSF20 ELISA Kit, ZTNF4 ELISA Kit, BAFF-R ELISA Kit, BAFFR ELISA Kit, BROMIX ELISA Kit, CD268 ELISA Kit, CVID4 ELISA Kit, prolixin ELISA Kit, TNFSF13B ELISA Kit, RGD1561519 ELISA Kit, RGD1560810 ELISA Kit, BLyS ELISA Kit, D8Ertd387e ELISA Kit, zTNF4 ELISA Kit, 2010006P15Rik ELISA Kit, Baffr ELISA Kit, Bcmd ELISA Kit, Bcmd-1 ELISA Kit, Bcmd1 ELISA Kit, Lvis22 ELISA Kit, zgc:172115 ELISA Kit, TNF superfamily member 13b ELISA Kit, TNF receptor superfamily member 13C ELISA Kit, tumor necrosis factor (ligand) superfamily, member 13b ELISA Kit, tumor necrosis factor receptor superfamily, member 13c ELISA Kit, tumor necrosis factor superfamily member 13b ELISA Kit, TNFSF13B ELISA Kit, TNFRSF13C ELISA Kit, Tnfsf13b ELISA Kit, Tnfrsf13c ELISA Kit, tnfsf13b ELISA Kit

Gene ID: 24099

NCBI Accession: NP_296371

UniProt: Q3KP92

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response