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TNFRSF1A ELISA Kit

TNFRSF1A Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Serum
Rockland
Catalog No. ABIN6719840
Supplier Product No.: koa0321
  • Target See all TNFRSF1A ELISA Kits
    TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
    Reactivity
    • 8
    • 8
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0321
    Supplier
    Rockland
    Purpose
    Mouse TNFR1 Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant mouse TNFR1. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 10 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product TNFRSF1A ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TNFR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse TNFR1 standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: L30-A212

    Endotoxin level: Low endotoxin

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all TNFRSF1A ELISA Kits
    TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
    Alternative Name
    TNFR1 (TNFRSF1A Products)
    Background

    Synonyms: CD120a, FPF, p55, p55 R, p60, p65, TBP1, TBPI, TNF R, TNF R I, TNF R55, TNF-R1, TNF-RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRI, TNFRSF1A, TNR1A_HUMAN, Tumor necrosis factor alpha receptor, Tumor necrosis factor binding protein 1, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor superfamily member 1A, membrane form, Tumor necrosis factor receptor type I, Tumor necrosis factor-binding protein 1

    Background: Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a, is a ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNF alpha). The protein encoded by this gene is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. And this protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate the transcription factor NF-kB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

    Gene ID
    21937
    NCBI Accession
    NP_035739
    UniProt
    P25118
    Pathways
    NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway
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