Kallikrein 11 ELISA Kit

Catalog No. ABIN6719900

Product Details Kallikrein 11 ELISA Kit

Target: Kallikrein 11 (KLK11)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Characteristics:

Synonyms: Hippostasin, hK11, Kallikrein 11, Kallikrein 11 isoform 1 preproprotein, Kallikrein 11 isoform 2 precursor, Kallikrein 11 precursor, Kallikrein related peptidase 11, Kallikrein-11 inactive chain 2, KLK 11, Klk11, KLK11_HUMAN, MGC33060, Protease serine 20 trypsin like, Protease serine trypsin like, PRSS20, Serine protease 20, TLSP, Trypsin like protease, Trypsin-like protease

Background: Kallikrein-11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

Gene Name: KLK11

Production: Natural and recombinant human KLK11. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: E19-N250

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK11 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Kallikrein 11

Target: Kallikrein 11 (KLK11)

Alternative Name: KLK11 (KLK11 Products)

Synonyms: KLK11 ELISA Kit, 2310015I08Rik ELISA Kit, Prss20 ELISA Kit, TLSP ELISA Kit, PRSS20 ELISA Kit, kallikrein related peptidase 11 ELISA Kit, kallikrein related-peptidase 11 ELISA Kit, kallikrein-related peptidase 11 ELISA Kit, KLK11 ELISA Kit, Klk11 ELISA Kit

Gene ID: 11012

NCBI Accession: NP_001129504

UniProt: Q9UBX7

Pathways: Complement System