Kallikrein 13 ELISA Kit (Kallikrein-Related Peptidase 13)

Details for Product KLK13 ELISA Kit No. ABIN6719902
Antigen
  • KLK-L4
  • KLKL4
  • Egfbp-2
  • mGk-13
  • kallikrein related peptidase 13
  • kallikrein related-peptidase 13
  • KLK13
  • Klk13
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
4
4
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
156 pg/mL - 10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Options
Purpose Sandwich ELISA for Quantitative Detection of Antigen
Sample Type Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
Analytical Method Quantitative
Detection Method Colorimetric
Characteristics

Synonyms: DKFZp586J1923, Kalikrein, Kallikrein 13, Kallikrein L4, Kallikrein Like 4, Kallikrein like 4 variant included, Kallikrein like gene 4, Kallikrein like protein 4, Kallikrein related peptidase 13, Kallikrein-13, Kallikrein-like protein 4, KLK-L4, KLK13, KLK13_HUMAN, KLKL 4, KLKL 4, KLKL4

Background: Kallikrein-13 is a protein that in humans is encoded by the KLK13 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. By genomic sequence analysis, KLK13 gene is mapped in a 300-kb region on chromosome 19q13.3-q13.4. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components. However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies.

Gene Name: KLK13

Production: Natural and recombinant human KLK13. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: G17-Q277

Plasmids, Primers & others Plasmids, Primers & others Kallikrein 13 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name KLK13 (KLK13 ELISA Kit Abstract)
Gene ID 26085
NCBI Accession NP_056411
UniProt Q9UKR3
Pathways Complement System
Application Notes

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK13 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Sample Volume 100 μL
Plate Pre-coated
Restrictions For Research Use only
Storage RT,4 °C,-20 °C
Storage Comment Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.