MSMB ELISA Kit

Catalog No. ABIN6720282

Supplier Product No.: koa0704

Product Details MSMB ELISA Kit

Target: MSMB (Microseminoprotein, beta (MSMB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Supplier Product No.: koa0704

Supplier: Rockland

Purpose: Human PSP94 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human PSP94. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human PSP94 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human PSP94 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: S21-I114

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for MSMB

Target: MSMB (Microseminoprotein, beta (MSMB))

Alternative Name: PSP94 (MSMB Products)

Background:

Synonyms: Beta microseminoprotein [Precursor], Beta-microseminoprotein, HPC 13, HPC13, IGBF, Immunoglobulin binding factor, Immunoglobulin-binding factor, microseminoprotein beta, Msmb, MSMB_HUMAN, MSP, MSPB, PN 44, PN44, Prostate secreted seminal plasma protein, Prostate secretory protein of 94 amino acids, Prostate secretory protein PSP94, PRPS, PRSP, PSP 57, PSP, PSP-94, PSP57, PSP94, Seminal plasma beta inhibin, Seminal plasma beta-inhibin

Background: Beta-microseminoprotein, also known as MSMB or PSP94, is a protein that in humans is encoded by the MSMB gene. It is a member of the immunoglobulin binding factor family. This gene is mapped to 10q11.23. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. The expression of the encoded protein is found to be decreased in prostate cancer. But transcripts of identical size were found in prostate, respiratory(tracheal, bronchial, and lung) tissues, and the mucosa of the gastric antrum. Thus, the protein may have functions other than those related to male reproduction. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues.

Gene ID: 4477

NCBI Accession: NP_002434

UniProt: P08118