CD45 ELISA Kit (Protein tyrosine Phosphatase, Receptor Type, C)

Details for Product PTPRC ELISA Kit No. ABIN6730447
Antigen
  • PTPRC
  • si:dkeyp-47c5.1
  • B220
  • CD45
  • CD45R
  • GP180
  • L-CA
  • LCA
  • LY5
  • T200
  • Cd45
  • Ly-5
  • Lyt-4
  • loc
  • Lca
  • RT7
  • cd45
  • protein tyrosine phosphatase, receptor type C
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • PTPRC
  • ptprc
  • LOC100074271
  • Ptprc
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
5
4
3
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.26 ng/mL - 15 ng/mL
Minimum Detection Limit
0.26 ng/mL
Application
ELISA
Options
Purpose Mouse CD45 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Sample Type Cell Culture Supernatant, Plasma, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA antibody pair recognizes Mouse CD45.
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others CD45 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD45 (PTPRC ELISA Kit Abstract)
UniProt P06800
Pathways TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events
Application Notes Optimal working dilution should be determined by the investigator.
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation

Recommended Dilution for serum and plasma samples2 fold

Restrictions For Research Use only
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
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