SLC3A2 ELISA Kit

Catalog No. ABIN6730512

Product Details SLC3A2 ELISA Kit

Target: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse SLC3A2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse SLC3A2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for SLC3A2

Target: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Alternative Name: SLC3A2 (SLC3A2 Products)

Synonyms: 4F2 ELISA Kit, 4F2HC ELISA Kit, 4T2HC ELISA Kit, CD98 ELISA Kit, CD98HC ELISA Kit, MDU1 ELISA Kit, NACAE ELISA Kit, Mdu1 ELISA Kit, MGC53951 ELISA Kit, slc3a2 ELISA Kit, MGC53965 ELISA Kit, SLC3A2 ELISA Kit, AI314110 ELISA Kit, Cd98 ELISA Kit, Ly-10 ELISA Kit, Ly-m10 ELISA Kit, Ly10 ELISA Kit, Mgp-2hc ELISA Kit, fc65h12 ELISA Kit, wu:fc65h12 ELISA Kit, id:ibd1327 ELISA Kit, sb:cb519 ELISA Kit, wu:fe14a07 ELISA Kit, wu:fq18d12 ELISA Kit, zgc:55813 ELISA Kit, solute carrier family 3 member 2 ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2 L homeolog ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2 S homeolog ELISA Kit, solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2a ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2b ELISA Kit, SLC3A2 ELISA Kit, Slc3a2 ELISA Kit, slc3a2.L ELISA Kit, slc3a2.S ELISA Kit, slc3a2a ELISA Kit, slc3a2b ELISA Kit

UniProt: P10852