Ubiquitin-Like 5 (UBL5) ELISA Kit

Details for Product No. ABIN6730525
Target
Reactivity
Human, Mouse, Rat
Alternatives
Kits with alternative reactivity to:
2
2
2
Detection Method
Colorimetric
Method Type
Competition ELISA
Detection Range
0.1 ng/mL - 1000 ng/mL
Minimum Detection Limit
0.1 ng/mL
Application
ELISA
Options
Purpose Human/Mouse/Rat Ubiquitin-like protein 5 EIA Kit optimized for serum and cell culture media. Competition-based ELISA on a 96-well strip plate.
Sample Type Cell Culture Supernatant, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This EIA kit is designed to detect human, mouse, and rat Ubiquitin-like protein 5
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Standard Peptide
  • Assay Diluent(s)
  • Biotinylated Peptide
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diagram
  • Positive Control Sample
  • Capture Antibody
  • User Manual
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Target
Alternative Name Ubiquitin-like protein 5 (UBL5 ELISA Kit Abstract)
Gene ID 500954
UniProt A9UMW0
Application Notes Optimal working dilution should be determined by the investigator.
Protocol
  1. Prepare all reagents, samples and standards as instructed.
  2. Add 100 μL detection antibody to each well.
  3. Incubate 1.5 h at RT or O/N at 4 °C.
  4. Add 100 μL standard or sample to each well.
  5. Incubate 2.5 h at RT.
  6. Add 100 μL prepared streptavidin solution.
  7. Incubate 45 min at RT.
  8. Add 100 μL TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL Stop Solution to each well.
  11. Read plate at 450 nm immediately.
Reagent Preparation

Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

Restrictions For Research Use only
Storage -20 °C
Storage Comment Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
Expiry Date 6 months
Images
Image no. 1 for Ubiquitin-Like 5 (UBL5) ELISA Kit (ABIN6730525) Standard Curve