WNT10B ELISA Kit (Wingless-Type MMTV Integration Site Family, Member 10B)

Details for Product WNT10B ELISA Kit No. ABIN6730526
Antigen
  • WNT10B
  • Xwnt-10b
  • Xwnt10b
  • wnt-12
  • Wnt12
  • SHFM6
  • WNT-12
  • Xwnt-10
  • wnt10
  • wnt10-A
  • Zwnt[d]
  • wnt10br
  • wnt[d]
  • Wnt family member 10B
  • wingless-type MMTV integration site family, member 10B
  • Wnt family member 10B S homeolog
  • wingless-type MMTV integration site family, member 10b
  • WNT10B
  • wnt10b
  • Wnt10b
  • wnt10b.S
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
5
4
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Purpose Mouse Wnt-10b ELISA Kit.
Sample Type Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA antibody pair recognizes Mouse Wnt-10b.
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others WNT10B products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Wnt-10b (WNT10B ELISA Kit Abstract)
UniProt P48614
Pathways WNT Signaling, Skeletal Muscle Fiber Development
Application Notes Optimal working dilution should be determined by the investigator.
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
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