Lysine (K)-Specific Demethylase 1A (KDM1A) ELISA Kit

Details for Product No. ABIN6730575
Antigen
  • AOF2
  • BHC110
  • KDM1
  • LSD1
  • 1810043O07Rik
  • AA408884
  • Aof2
  • D4Ertd478e
  • Kdm1
  • Lsd1
  • mKIAA0601
  • DKFZp459N2450
  • RGD1562975
  • aof2
  • kdm1
  • wu:fb82e06
  • wu:fc51a02
  • wu:fj36a05
  • wu:fj66b06
  • KDM1A
  • lsd1
  • xLSD1
  • lysine demethylase 1A
  • lysine (K)-specific demethylase 1A
  • lysine (K)-specific demethylase 1a
  • KDM1A
  • Kdm1a
  • kdm1a
Epitope
pSer112
Reactivity
Human, Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
4
2
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Purpose Human and Mouse Phospho-LSD1 (S112) and Total LSD1 ELISA Kit. This assay semi-quantitatively measures phosphorylated LSD1 (Ser112) and Total LSD1 in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity The antibody pair provided in this kit recognizes human and mouse LSD1 phosphorylated at Serine-112 and total LSD1.
Characteristics
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Antigen
Alternative Name LSD1 (KDM1A ELISA Kit Abstract)
Gene ID 23028
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
Supplier Images
Image no. 1 for Lysine (K)-Specific Demethylase 1A (KDM1A) ELISA Kit (ABIN6730575) Jurkat cells were treated with Calyculin A and Pervanadate. Cell lysates were analyze...
Image no. 2 for Lysine (K)-Specific Demethylase 1A (KDM1A) ELISA Kit (ABIN6730575) Jurkat cells were treated with Calyculin A and Pervanadate. Cell lysates were analyze...
Image no. 3 for Lysine (K)-Specific Demethylase 1A (KDM1A) ELISA Kit (ABIN6730575) Jurkat cells were treated with Calyculin A and Pervanadate. Solubilize cells at 4 x 1...
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