SEMA4D/CD100 ELISA Kit

Catalog No. ABIN6730590

Product Details SEMA4D/CD100 ELISA Kit

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 23 pg/mL - 5000 pg/mL

Minimum Detection Limit: 23 pg/mL

Application: ELISA

Purpose: Mouse Semaphorin 4D/CD100 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse Semaphorin 4D.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Reagent Preparation:

Recommended Dilution for serum and plasma samples15 fold

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for SEMA4D/CD100

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Alternative Name: Semaphorin 4D (CD100 (SEMA4D Products)

Synonyms: C9orf164 ELISA Kit, CD100 ELISA Kit, M-sema-G ELISA Kit, SEMAJ ELISA Kit, coll-4 ELISA Kit, Semacl2 ELISA Kit, Semaj ELISA Kit, Semcl2 ELISA Kit, COLL-4 ELISA Kit, semaphorin 4D ELISA Kit, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D ELISA Kit, Sema4d ELISA Kit, SEMA4D ELISA Kit

UniProt: O09126

Pathways: Regulation of Cell Size