SEMA4D ELISA Kit (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D)

Details for Product SEMA4D ELISA Kit No. ABIN6730590
Antigen
  • C9orf164
  • CD100
  • M-sema-G
  • SEMAJ
  • coll-4
  • Semacl2
  • Semaj
  • Semcl2
  • COLL-4
  • semaphorin 4D
  • sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
  • Sema4d
  • SEMA4D
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
6
4
3
2
2
2
2
2
2
2
1
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
23 pg/mL - 5000 pg/mL
Minimum Detection Limit
23 pg/mL
Application
ELISA
Options
Purpose Mouse Semaphorin 4D/CD100 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Sample Type Cell Culture Supernatant, Plasma, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA antibody pair recognizes Mouse Semaphorin 4D.
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others SEMA4D products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Semaphorin 4D (CD100 (SEMA4D ELISA Kit Abstract)
UniProt O09126
Pathways Regulation of Cell Size
Application Notes Optimal working dilution should be determined by the investigator.
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation

Recommended Dilution for serum and plasma samples15 fold

Restrictions For Research Use only
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
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