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BNIP3L/NIX ELISA Kit (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like) ELISA Kit

BNIP3L Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951385
$734.80
Plus shipping costs $45.00
96 tests
local_shipping Shipping to: United States
Delivery in 21 to 31 Business Days
  • Target
    BNIP3L/NIX (BNIP3L)
    Reactivity
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human BNIP3L ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target
    BNIP3L/NIX (BNIP3L)
    Alternative Name
    BNIP3L (BNIP3L ELISA Kit Abstract)
    Synonyms
    BNIP3a, NIX, Nix, Nip3, C86132, D14Ertd719e, Nip3L, bnip3l2, wu:fb52b03, wu:fc02h04, BCL2 interacting protein 3 like, BCL2/adenovirus E1B interacting protein 3, BCL2/adenovirus E1B interacting protein 3-like, BCL2/adenovirus E1B interacting protein 3-like a, BNIP3L, Bnip3l, Bnip3, bnip3la
    Gene ID
    665
    UniProt
    O60238
    Pathways
    Autophagy
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