+1 877 302 8632
+1 888 205 9894 (Toll-free)


PIIINP Reactivity: Human Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pubmed (30 references)
Catalog No. ABIN6958781
Plus shipping costs $45.00
Shipping to: United States
Delivery in 9 to 11 Business Days
  • Target See all PIIINP ELISA Kits
    PIIINP (Procollagen III N-Terminal Propeptide (PIIINP))
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Detection Method
    Method Type
    Sandwich ELISA
    Detection Range
    0.62 ng/mL - 40 ng/mL
    Minimum Detection Limit
    0.62 ng/mL
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PIIINP in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    This assay has high sensitivity and excellent specificity for detection of Procollagen III N-Terminal Propeptide (PIIINP)
    0.241 ng/mL
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
  • Comment

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Sample Volume
    100 μL
    Assay Time
    3 h
    1. Prepare all reagents, samples and standards,
    2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
    3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
    4. Aspirate and wash 3 times,
    5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    6. Aspirate and wash 5 times,
    7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    8. Add 50μL Stop Solution. Read at 450nm immediately.
    Reagent Preparation
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 160 ng/mL. Firstly dilute the stock solution to 40 ng/mL and the diluted standard serves as the highest standard (40 ng/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 40 ng/mL, 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.


    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    6. Contaminated water or container for reagent preparation will influence the detection result.
    Sample Preparation
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV < 10%
    Inter-Assay: CV < 12%
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    4 °C/-20 °C
    Storage Comment
    1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
    2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.
    Expiry Date
    6 months
  • Meel, Nethononda, Libhaber, Dix-Peek, Peters, Essop: "Assessment of myocardial fibrosis by late gadolinium enhancement imaging and biomarkers of collagen metabolism in chronic rheumatic mitral regurgitation." in: Cardiovascular journal of Africa, Vol. 29, Issue 3, pp. 150-154, (2019) (PubMed).

    Li, Zhang, Sun, Li, Yu, Fu, Chen: "Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia." in: Hernia : the journal of hernias and abdominal wall surgery, Vol. 22, Issue 3, pp. 541-547, (2019) (PubMed).

    Rubiś, Wiśniowska-Smiałek, Wypasek, Rudnicka-Sosin, Hlawaty, Leśniak-Sobelga, Kostkiewicz, Podolec et al.: "12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both ..." in: Cytokine, Vol. 96, pp. 217-227, (2018) (PubMed).

    Abdul Alim, Domeij-Arverud, Nilsson, Edman, Ackermann: "Achilles tendon rupture healing is enhanced by intermittent pneumatic compression upregulating collagen type I synthesis." in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, Vol. 26, Issue 7, pp. 2021-2029, (2018) (PubMed).

    Liu, Chen, Zhen, Ho, Tsang, Yuen, Lam, Tse, Yiu: "Relationship of biomarkers of extracellular matrix with myocardial function in Type 2 diabetes mellitus." in: Biomarkers in medicine, Vol. 11, Issue 7, pp. 569-578, (2018) (PubMed).

    Rubiś, Wiśniowska-Śmiałek, Dziewięcka, Rudnicka-Sosin, Kozanecki, Podolec: "Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events." in: Advances in medical sciences, Vol. 63, Issue 1, pp. 160-166, (2018) (PubMed).

    Sunman, Canpolat, Yorgun, Özkan, Yalçın, Bayrak, Şahiner, Kaya, Özkara, Aytemir, Oto: "Association between reverse electrical remodeling and cardiac fibrosis markers in patients with cardiac resynchronization therapy." in: Turk Kardiyoloji Dernegi arsivi : Turk Kardiyoloji Derneginin yayin organidir, Vol. 46, Issue 2, pp. 84-91, (2018) (PubMed).

    Jotwani, Katz, Ix, Gutiérrez, Bennett, Parikh, Cummings, Sarnak, Shlipak: "Urinary Biomarkers of Kidney Tubular Damage and Risk of Cardiovascular Disease and Mortality in Elders." in: American journal of kidney diseases : the official journal of the National Kidney Foundation, Vol. 72, Issue 2, pp. 205-213, (2018) (PubMed).

    Yılmaz, Yıldırım, Baydar, Altun, Erdem: "Urinary Type III Procollagen Is Associated With Chronic Allograft Dysfunction and Predicts Graft Survival." in: Transplantation proceedings, Vol. 49, Issue 2, pp. 281-287, (2017) (PubMed).

    Sokal, Lenarczyk, Kowalski, Mitrega, Pluta, Stabryla-Deska, Streb, Urbanik, Krzeminski, Kalarus: "Prognostic value of collagen turnover biomarkers in cardiac resynchronization therapy: A subanalysis of the TRUST CRT randomized trial population." in: Heart rhythm : the official journal of the Heart Rhythm Society, Vol. 13, Issue 5, pp. 1088-95, (2016) (PubMed).

    Rubiś, Wiśniowska-Śmialek, Wypasek, Biernacka-Fijalkowska, Rudnicka-Sosin, Dziewiecka, Faltyn, Khachatryan, Karabinowska, Kozanecki, Tomkiewicz-Pająk, Podolec: "Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 65, Issue 12, pp. 941-949, (2016) (PubMed).

    Lin, Kuo, Lee, Chou, Chen, Yeh, Huang, Hung, Liu, Ho, Wu: "5-methoxytryptophan is a potential marker for post-myocardial infarction heart failure - a preliminary approach to clinical utility." in: International journal of cardiology, Vol. 222, pp. 895-900, (2016) (PubMed).

    Rubiś, Wiśniowska-Śmiałek, Biernacka-Fijałkowska, Rudnicka-Sosin, Wypasek, Kozanecki, Dziewięcka, Faltyn, Karabinowska, Khachatryan, Hlawaty, Leśniak-Sobelga, Kostkiewicz, Płazak, Podolec: "Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR." in: Heart and vessels, Vol. 32, Issue 6, pp. 714-725, (2016) (PubMed).

    Buko, Lukivskaya, Naruta, Belonovskaya, Tauschel: "Protective Effects of Norursodeoxycholic Acid Versus Ursodeoxycholic Acid on Thioacetamide-induced Rat Liver Fibrosis." in: Journal of clinical and experimental hepatology, Vol. 4, Issue 4, pp. 293-301, (2015) (PubMed).

    Bielecka-Dabrowa, Michalska-Kasiczak, Gluba, Ahmed, Gerdts, von Haehling, Rysz, Banach: "Biomarkers and echocardiographic predictors of myocardial dysfunction in patients with hypertension." in: Scientific reports, Vol. 5, pp. 8916, (2015) (PubMed).

    Bielecka-Dabrowa, Gluba-Brzózka, Michalska-Kasiczak, Misztal, Rysz, Banach: "The multi-biomarker approach for heart failure in patients with hypertension." in: International journal of molecular sciences, Vol. 16, Issue 5, pp. 10715-33, (2015) (PubMed).

    Page, Charles, Piccinini, Nicolaidou, Taylor, Midwood: "Raised circulating tenascin-C in rheumatoid arthritis." in: Arthritis research & therapy, Vol. 14, Issue 6, pp. R260, (2014) (PubMed).

    Durnea, Khashan, Kenny, Durnea, Smyth, OReilly: "Prevalence, etiology and risk factors of pelvic organ prolapse in premenopausal primiparous women." in: International urogynecology journal, Vol. 25, Issue 11, pp. 1463-70, (2014) (PubMed).

    Banerjee, Mukherjee, Ghosh, Biswas, Dutta, Pattari, Chatterjee, Bandyopadhyay: "Clinical significance of markers of collagen metabolism in rheumatic mitral valve disease." in: PLoS ONE, Vol. 9, Issue 3, pp. e90527, (2014) (PubMed).

    Jianguo, Zhenzhen, Xianghua, Zhanzheng, Suke, Suyun: "Serum and urinary procollagen III aminoterminal propeptide as a biomarker of obstructive nephropathy in children." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 434, pp. 29-33, (2014) (PubMed).

  • Target See all PIIINP ELISA Kits
    PIIINP (Procollagen III N-Terminal Propeptide (PIIINP))
    Alternative Name
    Procollagen III Propeptide (PIIINP) (PIIINP Products)
You are here: