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ATP6V0A2 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN6975490
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  • Target See all ATP6V0A2 ELISA Kits
    ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))
    Detection Method
    Method Type
    Sandwich ELISA
    Detection Range
    31.25 pg/mL - 2000 pg/mL
    Minimum Detection Limit
    31.25 pg/mL
    For the quantitative determination of human V-type proton ATPase 116 kDa subunit a isoform 2 (ATP6V0A2) concentrations in serum, plasma, tissue homogenates.
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    This assay has high sensitivity and excellent specificity for detection of human ATP6V0A2. No significant cross-reactivity or interference between human ATP6V0A2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human ATP6V0A2 and all the analogues, therefore, cross reaction may still exist.
    7.81 pg/mL
    • Assay plate
    • Standard
    • HRP-avidin (100 x concentrate)
    • Biotin-antibody (100 x concentrate)
    • Sample Diluent
    • HRP-avidin Diluent
    • Biotin-antibody Diluent
    • Wash Buffer (25 x concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Sample Volume
    100 μL
    Assay Time
    1 - 4.5 h
    1. Prepare reagents, samples and standards as instructed.
    2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C.
    3. Remove the liquid of each well, don't wash.
    4. Add 100 µL Biotin-antibody (1x) to each well. Incubate 1 hour at 37 °C.
    5. Aspirate and wash 3 times.
    6. Add 100 µL HRP-avidin (1x) to each well. Incubate 1 hour at 37 °C
    7. Aspirate and wash 5 times.
    8. Add 90 μL of TMB Substrate to each well. Incubate for 15-30 minutes at 37 °C. Protect from light.
    9. Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.
    Reagent Preparation
    1. Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
    2. HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
    3. Wash Buffer(1x)- If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
    4. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 2000 pg/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (2000 pg/mL). Sample Diluent serves as the zero standard (0 pg/mL).
    • Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
    • Bring all reagents to room temperature (18-25 °C) before use for 30 min.
    • Prepare fresh standard for each assay. Use within 4 hours and discard after use.
    • Making serial dilution in the wells directly is not permitted.
    • Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
    • Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.
    Sample Preparation
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    Assay Precision
    Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
    Expiry Date
    6 months
  • Target See all ATP6V0A2 ELISA Kits
    ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))
    Alternative Name
    ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2 Products)
    A2 ELISA Kit, ARCL ELISA Kit, ARCL2A ELISA Kit, ATP6A2 ELISA Kit, ATP6N1D ELISA Kit, J6B7 ELISA Kit, RTF ELISA Kit, STV1 ELISA Kit, TJ6 ELISA Kit, TJ6M ELISA Kit, TJ6S ELISA Kit, VPH1 ELISA Kit, WSS ELISA Kit, 8430408C20Rik ELISA Kit, AI385560 ELISA Kit, ATP6a2 ELISA Kit, AW489264 ELISA Kit, Atp6n1d ELISA Kit, Atp6n2 ELISA Kit, C76904 ELISA Kit, ISF ELISA Kit, SHIF ELISA Kit, Stv1 ELISA Kit, TJ6s ELISA Kit, Tj6 ELISA Kit, V-ATPase 116 kDa ELISA Kit, V-ATPase a2 ELISA Kit, Cc1-3 ELISA Kit, J6b7 ELISA Kit, atp6v0a2 ELISA Kit, si:ch211-199i18.4 ELISA Kit, si:ch211-106a19.2 ELISA Kit, ATPase H+ transporting V0 subunit a2 ELISA Kit, ATPase, H+ transporting, lysosomal V0 subunit A2 ELISA Kit, ATPase, H+ transporting, lysosomal V0 subunit a2a ELISA Kit, ATPase, H+ transporting, lysosomal V0 subunit a2b ELISA Kit, ATP6V0A2 ELISA Kit, Atp6v0a2 ELISA Kit, atp6v0a2a ELISA Kit, atp6v0a2b ELISA Kit

    Abbreviation: ATP6V0A2

    Alias: A2, ARCL, ATP6A2, ATP6N1D, J6B7, RTF, STV1, TJ6, TJ6M, TJ6S, VPH1, WSS, A2V-ATPase|ATPase, H+ transporting, lysosomal V0 subunit A2|regeneration and tolerance factor|v-ATPase 116 kDa|v-type proton A

    Transition Metal Ion Homeostasis, Proton Transport
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